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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP388200RIJ
CSB-EP388200RIJ
CSB-BP388200RIJ
CSB-MP388200RIJ
CSB-EP388200RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP388201RIJ
CSB-EP388201RIJ
CSB-BP388201RIJ
CSB-MP388201RIJ
CSB-EP388201RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP388202RIJ
CSB-EP388202RIJ
CSB-BP388202RIJ
CSB-MP388202RIJ
CSB-EP388202RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP388203RIJ
CSB-EP388203RIJ
CSB-BP388203RIJ
CSB-MP388203RIJ
CSB-EP388203RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit beta 1 (atpD1) CSB-YP388204RIJ
CSB-EP388204RIJ
CSB-BP388204RIJ
CSB-MP388204RIJ
CSB-EP388204RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Xanthine phosphoribosyltransferase (gpt) CSB-YP388205RIJ
CSB-EP388205RIJ
CSB-BP388205RIJ
CSB-MP388205RIJ
CSB-EP388205RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP388206RIJ
CSB-EP388206RIJ
CSB-BP388206RIJ
CSB-MP388206RIJ
CSB-EP388206RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphoglycerate kinase (pgk) CSB-YP388207RIJ
CSB-EP388207RIJ
CSB-BP388207RIJ
CSB-MP388207RIJ
CSB-EP388207RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP388208RIJ
CSB-EP388208RIJ
CSB-BP388208RIJ
CSB-MP388208RIJ
CSB-EP388208RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit I 1 (nuoI1) CSB-YP388209RIJ
CSB-EP388209RIJ
CSB-BP388209RIJ
CSB-MP388209RIJ
CSB-EP388209RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP388210RIJ
CSB-EP388210RIJ
CSB-BP388210RIJ
CSB-MP388210RIJ
CSB-EP388210RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP388211RIJ
CSB-EP388211RIJ
CSB-BP388211RIJ
CSB-MP388211RIJ
CSB-EP388211RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP388212RIJ
CSB-EP388212RIJ
CSB-BP388212RIJ
CSB-MP388212RIJ
CSB-EP388212RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glycerol kinase (glpK) CSB-YP388213RIJ
CSB-EP388213RIJ
CSB-BP388213RIJ
CSB-MP388213RIJ
CSB-EP388213RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP388214RIJ
CSB-EP388214RIJ
CSB-BP388214RIJ
CSB-MP388214RIJ
CSB-EP388214RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Cysteine--tRNA ligase (cysS) CSB-YP388215RIJ
CSB-EP388215RIJ
CSB-BP388215RIJ
CSB-MP388215RIJ
CSB-EP388215RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S2 (rpsB) CSB-YP388216RIJ
CSB-EP388216RIJ
CSB-BP388216RIJ
CSB-MP388216RIJ
CSB-EP388216RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-dehydroquinate synthase (aroB) CSB-YP388217RIJ
CSB-EP388217RIJ
CSB-BP388217RIJ
CSB-MP388217RIJ
CSB-EP388217RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP388218RIJ
CSB-EP388218RIJ
CSB-BP388218RIJ
CSB-MP388218RIJ
CSB-EP388218RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP388219RIJ
CSB-EP388219RIJ
CSB-BP388219RIJ
CSB-MP388219RIJ
CSB-EP388219RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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