Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharopolyspora erythraea Aspartate carbamoyltransferase (pyrB) CSB-YP389202STB
CSB-EP389202STB
CSB-BP389202STB
CSB-MP389202STB
CSB-EP389202STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Putative sporulation transcription regulator WhiA (whiA) CSB-YP389203STB
CSB-EP389203STB
CSB-BP389203STB
CSB-MP389203STB
CSB-EP389203STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea ATP phosphoribosyltransferase (hisG) CSB-YP389204STB
CSB-EP389204STB
CSB-BP389204STB
CSB-MP389204STB
CSB-EP389204STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Prokaryotic ubiquitin-like protein Pup 1 (pup1) CSB-YP389205STB
CSB-EP389205STB
CSB-BP389205STB
CSB-MP389205STB
CSB-EP389205STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Carboxylate-amine ligase SACE_2909 (SACE_2909) CSB-YP389206STB
CSB-EP389206STB
CSB-BP389206STB
CSB-MP389206STB
CSB-EP389206STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Acireductone dioxygenase (mtnD) CSB-YP389207STB
CSB-EP389207STB
CSB-BP389207STB
CSB-MP389207STB
CSB-EP389207STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 2-methylisoborneol synthase (SACE_3722) CSB-YP389208STB
CSB-EP389208STB
CSB-BP389208STB
CSB-MP389208STB
CSB-EP389208STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Inositol 2-dehydrogenase 2 (iolG2) CSB-YP389209STB
CSB-EP389209STB
CSB-BP389209STB
CSB-MP389209STB
CSB-EP389209STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Inositol 2-dehydrogenase 4 (iolG4) CSB-YP389210STB
CSB-EP389210STB
CSB-BP389210STB
CSB-MP389210STB
CSB-EP389210STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L35 (rpmI) CSB-YP389211STB
CSB-EP389211STB
CSB-BP389211STB
CSB-MP389211STB
CSB-EP389211STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP389212STB
CSB-EP389212STB
CSB-BP389212STB
CSB-MP389212STB
CSB-EP389212STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea L-rhamnonate dehydratase (rhmD) CSB-YP389213STB
CSB-EP389213STB
CSB-BP389213STB
CSB-MP389213STB
CSB-EP389213STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Protein MraZ (mraZ) CSB-YP389214STB
CSB-EP389214STB
CSB-BP389214STB
CSB-MP389214STB
CSB-EP389214STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP389215STB
CSB-EP389215STB
CSB-BP389215STB
CSB-MP389215STB
CSB-EP389215STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Ribonuclease HII (rnhB) CSB-YP389216STB
CSB-EP389216STB
CSB-BP389216STB
CSB-MP389216STB
CSB-EP389216STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea tRNA (guanine-N (1)-)-methyltransferase CSB-YP389217STB
CSB-EP389217STB
CSB-BP389217STB
CSB-MP389217STB
CSB-EP389217STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP389218STB
CSB-EP389218STB
CSB-BP389218STB
CSB-MP389218STB
CSB-EP389218STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea DNA ligase (ligA), partial CSB-YP389219STB
CSB-EP389219STB
CSB-BP389219STB
CSB-MP389219STB
CSB-EP389219STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea ATP synthase gamma chain (atpG) CSB-YP389220STB
CSB-EP389220STB
CSB-BP389220STB
CSB-MP389220STB
CSB-EP389220STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L31 (rpmE) CSB-YP389221STB
CSB-EP389221STB
CSB-BP389221STB
CSB-MP389221STB
CSB-EP389221STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>