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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Herminiimonas arsenicoxydans Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP390002HSJ
CSB-EP390002HSJ
CSB-BP390002HSJ
CSB-MP390002HSJ
CSB-EP390002HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP390003HSJ
CSB-EP390003HSJ
CSB-BP390003HSJ
CSB-MP390003HSJ
CSB-EP390003HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0061 protein HEAR1735 (HEAR1735) CSB-YP390004HSJ
CSB-EP390004HSJ
CSB-BP390004HSJ
CSB-MP390004HSJ
CSB-EP390004HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP390005HSJ
CSB-EP390005HSJ
CSB-BP390005HSJ
CSB-MP390005HSJ
CSB-EP390005HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP390006HSJ
CSB-EP390006HSJ
CSB-BP390006HSJ
CSB-MP390006HSJ
CSB-EP390006HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar L-ring protein (flgH) CSB-YP390007HSJ
CSB-EP390007HSJ
CSB-BP390007HSJ
CSB-MP390007HSJ
CSB-EP390007HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)/H (+) antiporter nhaA, partial CSB-YP390008HSJ
CSB-EP390008HSJ
CSB-BP390008HSJ
CSB-MP390008HSJ
CSB-EP390008HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S6 (rpsF) CSB-YP390009HSJ
CSB-EP390009HSJ
CSB-BP390009HSJ
CSB-MP390009HSJ
CSB-EP390009HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase (eno) CSB-YP390010HSJ
CSB-EP390010HSJ
CSB-BP390010HSJ
CSB-MP390010HSJ
CSB-EP390010HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein hscA homolog (hscA), partial CSB-YP390011HSJ
CSB-EP390011HSJ
CSB-BP390011HSJ
CSB-MP390011HSJ
CSB-EP390011HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor NrdR (nrdR) CSB-YP390012HSJ
CSB-EP390012HSJ
CSB-BP390012HSJ
CSB-MP390012HSJ
CSB-EP390012HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP390013HSJ
CSB-EP390013HSJ
CSB-BP390013HSJ
CSB-MP390013HSJ
CSB-EP390013HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP390014HSJ
CSB-EP390014HSJ
CSB-BP390014HSJ
CSB-MP390014HSJ
CSB-EP390014HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0434 protein HEAR2489 (HEAR2489) CSB-YP390015HSJ
CSB-EP390015HSJ
CSB-BP390015HSJ
CSB-MP390015HSJ
CSB-EP390015HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP390016HSJ
CSB-EP390016HSJ
CSB-BP390016HSJ
CSB-MP390016HSJ
CSB-EP390016HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP390017HSJ
CSB-EP390017HSJ
CSB-BP390017HSJ
CSB-MP390017HSJ
CSB-EP390017HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP390018HSJ1
CSB-EP390018HSJ1
CSB-BP390018HSJ1
CSB-MP390018HSJ1
CSB-EP390018HSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP390019HSJ
CSB-EP390019HSJ
CSB-BP390019HSJ
CSB-MP390019HSJ
CSB-EP390019HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecA (secA), partial CSB-YP390020HSJ
CSB-EP390020HSJ
CSB-BP390020HSJ
CSB-MP390020HSJ
CSB-EP390020HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP390021HSJ
CSB-EP390021HSJ
CSB-BP390021HSJ
CSB-MP390021HSJ
CSB-EP390021HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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