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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium sp. Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP388601MOL
CSB-EP388601MOL
CSB-BP388601MOL
CSB-MP388601MOL
CSB-EP388601MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Uncharacterized tRNA/rRNA methyltransferase Mjls_5122 (Mjls_5122) CSB-YP388602MOL
CSB-EP388602MOL
CSB-BP388602MOL
CSB-MP388602MOL
CSB-EP388602MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Aspartate 1-decarboxylase (panD) CSB-YP388603MOL
CSB-EP388603MOL
CSB-BP388603MOL
CSB-MP388603MOL
CSB-EP388603MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Pantothenate synthetase (panC) CSB-YP388604MOL
CSB-EP388604MOL
CSB-BP388604MOL
CSB-MP388604MOL
CSB-EP388604MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Serine--tRNA ligase (serS) CSB-YP388605MOL
CSB-EP388605MOL
CSB-BP388605MOL
CSB-MP388605MOL
CSB-EP388605MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP388606MOL
CSB-EP388606MOL
CSB-BP388606MOL
CSB-MP388606MOL
CSB-EP388606MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (Mjls_5511) CSB-YP388607MOL
CSB-EP388607MOL
CSB-BP388607MOL
CSB-MP388607MOL
CSB-EP388607MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S18 (rpsR) CSB-YP388608MOL
CSB-EP388608MOL
CSB-BP388608MOL
CSB-MP388608MOL
CSB-EP388608MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L34 (rpmH) CSB-YP388609MOL
CSB-EP388609MOL
CSB-BP388609MOL
CSB-MP388609MOL
CSB-EP388609MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Sulfurtransferase TusA homolog (tusA) CSB-YP388610STO
CSB-EP388610STO
CSB-BP388610STO
CSB-MP388610STO
CSB-EP388610STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP388611STO
CSB-EP388611STO
CSB-BP388611STO
CSB-MP388611STO
CSB-EP388611STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Fe/S biogenesis protein NfuA (nfuA) CSB-YP388612STO
CSB-EP388612STO
CSB-BP388612STO
CSB-MP388612STO
CSB-EP388612STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glutamate racemase (murI) CSB-YP388613STO
CSB-EP388613STO
CSB-BP388613STO
CSB-MP388613STO
CSB-EP388613STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Pantothenate kinase (coaA) CSB-YP388614STO
CSB-EP388614STO
CSB-BP388614STO
CSB-MP388614STO
CSB-EP388614STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L10 (rplJ) CSB-YP388615STO
CSB-EP388615STO
CSB-BP388615STO
CSB-MP388615STO
CSB-EP388615STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S7 (rpsG) CSB-YP388616STO
CSB-EP388616STO
CSB-BP388616STO
CSB-MP388616STO
CSB-EP388616STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L4 (rplD) CSB-YP388617STO
CSB-EP388617STO
CSB-BP388617STO
CSB-MP388617STO
CSB-EP388617STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L2 (rplB) CSB-YP388618STO
CSB-EP388618STO
CSB-BP388618STO
CSB-MP388618STO
CSB-EP388618STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S3 (rpsC) CSB-YP388619STO
CSB-EP388619STO
CSB-BP388619STO
CSB-MP388619STO
CSB-EP388619STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S8 (rpsH) CSB-YP388620STO
CSB-EP388620STO
CSB-BP388620STO
CSB-MP388620STO
CSB-EP388620STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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