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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP389802MNP
CSB-EP389802MNP
CSB-BP389802MNP
CSB-MP389802MNP
CSB-EP389802MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Homoserine kinase (thrB) CSB-YP389803MNP
CSB-EP389803MNP
CSB-BP389803MNP
CSB-MP389803MNP
CSB-EP389803MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA-directed RNA polymerase subunit L (rpoL) CSB-YP389804MNP
CSB-EP389804MNP
CSB-BP389804MNP
CSB-MP389804MNP
CSB-EP389804MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L37Ae (rpl37Ae) CSB-YP389805MNP
CSB-EP389805MNP
CSB-BP389805MNP
CSB-MP389805MNP
CSB-EP389805MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP389806MNP
CSB-EP389806MNP
CSB-BP389806MNP
CSB-MP389806MNP
CSB-EP389806MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP389807MNP
CSB-EP389807MNP
CSB-BP389807MNP
CSB-MP389807MNP
CSB-EP389807MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hypoxanthine/guanine phosphoribosyltransferase (hpt) CSB-YP389808MNP
CSB-EP389808MNP
CSB-BP389808MNP
CSB-MP389808MNP
CSB-EP389808MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP389809MNP
CSB-EP389809MNP
CSB-BP389809MNP
CSB-MP389809MNP
CSB-EP389809MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L21e (rpl21e) CSB-YP389810MNP
CSB-EP389810MNP
CSB-BP389810MNP
CSB-MP389810MNP
CSB-EP389810MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable DNA primase small subunit (priA) CSB-YP389811MNP
CSB-EP389811MNP
CSB-BP389811MNP
CSB-MP389811MNP
CSB-EP389811MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein LX (rplX) CSB-YP389812MNP
CSB-EP389812MNP
CSB-BP389812MNP
CSB-MP389812MNP
CSB-EP389812MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP389813MNP
CSB-EP389813MNP
CSB-BP389813MNP
CSB-MP389813MNP
CSB-EP389813MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial CSB-YP389814MNP
CSB-EP389814MNP
CSB-BP389814MNP
CSB-MP389814MNP
CSB-EP389814MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 3-dehydroquinate synthase (MmarC5_1678) CSB-YP389815MNP
CSB-EP389815MNP
CSB-BP389815MNP
CSB-MP389815MNP
CSB-EP389815MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribosome biogenesis protein Nop10 (MmarC5_1700) CSB-YP389816MNP
CSB-EP389816MNP
CSB-BP389816MNP
CSB-MP389816MNP
CSB-EP389816MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable tRNA pseudouridine synthase B (truB) CSB-YP389817MNP
CSB-EP389817MNP
CSB-BP389817MNP
CSB-MP389817MNP
CSB-EP389817MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP389818MNP
CSB-EP389818MNP
CSB-BP389818MNP
CSB-MP389818MNP
CSB-EP389818MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartate--tRNA ligase (aspS) CSB-YP389819MNP
CSB-EP389819MNP
CSB-BP389819MNP
CSB-MP389819MNP
CSB-EP389819MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP389820MNP
CSB-EP389820MNP
CSB-BP389820MNP
CSB-MP389820MNP
CSB-EP389820MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA) CSB-YP389821HSJ
CSB-EP389821HSJ
CSB-BP389821HSJ
CSB-MP389821HSJ
CSB-EP389821HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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