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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacillus thermodenitrificans Probable malate:quinone oxidoreductase (mqo), partial CSB-YP390162GEH
CSB-EP390162GEH
CSB-BP390162GEH
CSB-MP390162GEH
CSB-EP390162GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP390163GEH
CSB-EP390163GEH
CSB-BP390163GEH
CSB-MP390163GEH
CSB-EP390163GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Hydroxyethylthiazole kinase (thiM) CSB-YP390164GEH
CSB-EP390164GEH
CSB-BP390164GEH
CSB-MP390164GEH
CSB-EP390164GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Malate synthase G (glcB), partial CSB-YP390165GEH
CSB-EP390165GEH
CSB-BP390165GEH
CSB-MP390165GEH
CSB-EP390165GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Small, acid-soluble spore protein tlp (tlp) CSB-YP390166GEH
CSB-EP390166GEH
CSB-BP390166GEH
CSB-MP390166GEH
CSB-EP390166GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Peptidase T (pepT) CSB-YP390167GEH
CSB-EP390167GEH
CSB-BP390167GEH
CSB-MP390167GEH
CSB-EP390167GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Xylose isomerase (xylA) CSB-YP390168GEH
CSB-EP390168GEH
CSB-BP390168GEH
CSB-MP390168GEH
CSB-EP390168GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP390169GEH
CSB-EP390169GEH
CSB-BP390169GEH
CSB-MP390169GEH
CSB-EP390169GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP390170GEH
CSB-EP390170GEH
CSB-BP390170GEH
CSB-MP390170GEH
CSB-EP390170GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, partial CSB-YP390171GEH
CSB-EP390171GEH
CSB-BP390171GEH
CSB-MP390171GEH
CSB-EP390171GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Methylmalonate semialdehyde dehydrogenase [acylating] 2 (iolA2) CSB-YP390172GEH
CSB-EP390172GEH
CSB-BP390172GEH
CSB-MP390172GEH
CSB-EP390172GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Shikimate kinase (aroK) CSB-YP390173GEH
CSB-EP390173GEH
CSB-BP390173GEH
CSB-MP390173GEH
CSB-EP390173GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Aspartate 1-decarboxylase (panD) CSB-YP390174GEH
CSB-EP390174GEH
CSB-BP390174GEH
CSB-MP390174GEH
CSB-EP390174GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Pantothenate synthetase (panC) CSB-YP390175GEH
CSB-EP390175GEH
CSB-BP390175GEH
CSB-MP390175GEH
CSB-EP390175GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Dihydrodipicolinate reductase (dapB) CSB-YP390176GEH
CSB-EP390176GEH
CSB-BP390176GEH
CSB-MP390176GEH
CSB-EP390176GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Tryptophan synthase beta chain (trpB) CSB-YP390177GEH
CSB-EP390177GEH
CSB-BP390177GEH
CSB-MP390177GEH
CSB-EP390177GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Chorismate synthase (aroC) CSB-YP390178GEH
CSB-EP390178GEH
CSB-BP390178GEH
CSB-MP390178GEH
CSB-EP390178GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Cytidylate kinase (cmk) CSB-YP390179GEH
CSB-EP390179GEH
CSB-BP390179GEH
CSB-MP390179GEH
CSB-EP390179GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Arginine repressor (argR) CSB-YP390180GEH
CSB-EP390180GEH
CSB-BP390180GEH
CSB-MP390180GEH
CSB-EP390180GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Bifunctional protein FolD (folD) CSB-YP390181GEH
CSB-EP390181GEH
CSB-BP390181GEH
CSB-MP390181GEH
CSB-EP390181GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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