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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia vietnamiensis Urease subunit alpha (ureC), partial CSB-YP390362BPV
CSB-EP390362BPV
CSB-BP390362BPV
CSB-MP390362BPV
CSB-EP390362BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Urease subunit gamma (ureA) CSB-YP390363BPV
CSB-EP390363BPV
CSB-BP390363BPV
CSB-MP390363BPV
CSB-EP390363BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Protein CrcB homolog (crcB), partial CSB-YP390364BPV1
CSB-EP390364BPV1
CSB-BP390364BPV1
CSB-MP390364BPV1
CSB-EP390364BPV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Serine--tRNA ligase (serS) CSB-YP390365BPV
CSB-EP390365BPV
CSB-BP390365BPV
CSB-MP390365BPV
CSB-EP390365BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis D-amino acid dehydrogenase small subunit (dadA) CSB-YP390366BPV
CSB-EP390366BPV
CSB-BP390366BPV
CSB-MP390366BPV
CSB-EP390366BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Elongation factor 4 (lepA), partial CSB-YP390367BPV
CSB-EP390367BPV
CSB-BP390367BPV
CSB-MP390367BPV
CSB-EP390367BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP390368BPV
CSB-EP390368BPV
CSB-BP390368BPV
CSB-MP390368BPV
CSB-EP390368BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphoglucosamine mutase (glmM) CSB-YP390369BPV
CSB-EP390369BPV
CSB-BP390369BPV
CSB-MP390369BPV
CSB-EP390369BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L35 (rpmI) CSB-YP390370BPV
CSB-EP390370BPV
CSB-BP390370BPV
CSB-MP390370BPV
CSB-EP390370BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP390371BPV
CSB-EP390371BPV
CSB-BP390371BPV
CSB-MP390371BPV
CSB-EP390371BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Ribosome-binding factor A (rbfA) CSB-YP390372BPV
CSB-EP390372BPV
CSB-BP390372BPV
CSB-MP390372BPV
CSB-EP390372BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0303 protein Bcep1808_1522 (Bcep1808_1522) CSB-YP390373BPV
CSB-EP390373BPV
CSB-BP390373BPV
CSB-MP390373BPV
CSB-EP390373BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP390374BPV
CSB-EP390374BPV
CSB-BP390374BPV
CSB-MP390374BPV
CSB-EP390374BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP390375BPV
CSB-EP390375BPV
CSB-BP390375BPV
CSB-MP390375BPV
CSB-EP390375BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Histidine--tRNA ligase (hisS) CSB-YP390376BPV
CSB-EP390376BPV
CSB-BP390376BPV
CSB-MP390376BPV
CSB-EP390376BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP390377BPV
CSB-EP390377BPV
CSB-BP390377BPV
CSB-MP390377BPV
CSB-EP390377BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0133 protein Bcep1808_1753 (Bcep1808_1753) CSB-YP390378BPV
CSB-EP390378BPV
CSB-BP390378BPV
CSB-MP390378BPV
CSB-EP390378BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S18 (rpsR) CSB-YP390379BPV
CSB-EP390379BPV
CSB-BP390379BPV
CSB-MP390379BPV
CSB-EP390379BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0061 protein Bcep1808_1840 (Bcep1808_1840), partial CSB-YP390380BPV
CSB-EP390380BPV
CSB-BP390380BPV
CSB-MP390380BPV
CSB-EP390380BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP390381BPV
CSB-EP390381BPV
CSB-BP390381BPV
CSB-MP390381BPV
CSB-EP390381BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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