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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prosthecochloris vibrioformis Zinc transporter ZupT (zupT), partial CSB-YP390742PZJ
CSB-EP390742PZJ
CSB-BP390742PZJ
CSB-MP390742PZJ
CSB-EP390742PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP390743PZJ
CSB-EP390743PZJ
CSB-BP390743PZJ
CSB-MP390743PZJ
CSB-EP390743PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP390744PZJ
CSB-EP390744PZJ
CSB-BP390744PZJ
CSB-MP390744PZJ
CSB-EP390744PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Large-conductance mechanosensitive channel (mscL), partial CSB-YP390745PZJ1
CSB-EP390745PZJ1
CSB-BP390745PZJ1
CSB-MP390745PZJ1
CSB-EP390745PZJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Ribosome maturation factor RimM (rimM) CSB-YP390746PZJ
CSB-EP390746PZJ
CSB-BP390746PZJ
CSB-MP390746PZJ
CSB-EP390746PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Adenylate kinase (adk) CSB-YP390747PZJ
CSB-EP390747PZJ
CSB-BP390747PZJ
CSB-MP390747PZJ
CSB-EP390747PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis UPF0133 protein Cvib_1034 (Cvib_1034) CSB-YP390748PZJ
CSB-EP390748PZJ
CSB-BP390748PZJ
CSB-MP390748PZJ
CSB-EP390748PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP390749PZJ
CSB-EP390749PZJ
CSB-BP390749PZJ
CSB-MP390749PZJ
CSB-EP390749PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP390750PZJ
CSB-EP390750PZJ
CSB-BP390750PZJ
CSB-MP390750PZJ
CSB-EP390750PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Ketol-acid reductoisomerase (ilvC) CSB-YP390751PZJ
CSB-EP390751PZJ
CSB-BP390751PZJ
CSB-MP390751PZJ
CSB-EP390751PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP390752PZJ
CSB-EP390752PZJ
CSB-BP390752PZJ
CSB-MP390752PZJ
CSB-EP390752PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Transcription elongation factor GreA (greA) CSB-YP390753PZJ
CSB-EP390753PZJ
CSB-BP390753PZJ
CSB-MP390753PZJ
CSB-EP390753PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Elongation factor 4 (lepA), partial CSB-YP390754PZJ
CSB-EP390754PZJ
CSB-BP390754PZJ
CSB-MP390754PZJ
CSB-EP390754PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Protein grpE (grpE) CSB-YP390755PZJ
CSB-EP390755PZJ
CSB-BP390755PZJ
CSB-MP390755PZJ
CSB-EP390755PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Heat-inducible transcription repressor HrcA (hrcA) CSB-YP390756PZJ
CSB-EP390756PZJ
CSB-BP390756PZJ
CSB-MP390756PZJ
CSB-EP390756PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 50S ribosomal protein L21 (rplU) CSB-YP390757PZJ
CSB-EP390757PZJ
CSB-BP390757PZJ
CSB-MP390757PZJ
CSB-EP390757PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP390758PZJ
CSB-EP390758PZJ
CSB-BP390758PZJ
CSB-MP390758PZJ
CSB-EP390758PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis HPr kinase/phosphorylase (hprK) CSB-YP390759PZJ
CSB-EP390759PZJ
CSB-BP390759PZJ
CSB-MP390759PZJ
CSB-EP390759PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP390760PZJ
CSB-EP390760PZJ
CSB-BP390760PZJ
CSB-MP390760PZJ
CSB-EP390760PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP390761PZJ
CSB-EP390761PZJ
CSB-BP390761PZJ
CSB-MP390761PZJ
CSB-EP390761PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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