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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S8 (rpsH) CSB-YP390342BPV
CSB-EP390342BPV
CSB-BP390342BPV
CSB-MP390342BPV
CSB-EP390342BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L6 (rplF) CSB-YP390343BPV
CSB-EP390343BPV
CSB-BP390343BPV
CSB-MP390343BPV
CSB-EP390343BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L15 (rplO) CSB-YP390344BPV
CSB-EP390344BPV
CSB-BP390344BPV
CSB-MP390344BPV
CSB-EP390344BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S11 (rpsK) CSB-YP390345BPV
CSB-EP390345BPV
CSB-BP390345BPV
CSB-MP390345BPV
CSB-EP390345BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP390346BPV
CSB-EP390346BPV
CSB-BP390346BPV
CSB-MP390346BPV
CSB-EP390346BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP390347BPV
CSB-EP390347BPV
CSB-BP390347BPV
CSB-MP390347BPV
CSB-EP390347BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Protein MraZ (mraZ) CSB-YP390348BPV
CSB-EP390348BPV
CSB-BP390348BPV
CSB-MP390348BPV
CSB-EP390348BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP390349BPV
CSB-EP390349BPV
CSB-BP390349BPV
CSB-MP390349BPV
CSB-EP390349BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP390350BPV
CSB-EP390350BPV
CSB-BP390350BPV
CSB-MP390350BPV
CSB-EP390350BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L21 (rplU) CSB-YP390351BPV
CSB-EP390351BPV
CSB-BP390351BPV
CSB-MP390351BPV
CSB-EP390351BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Ribosomal protein L11 methyltransferase (prmA) CSB-YP390352BPV
CSB-EP390352BPV
CSB-BP390352BPV
CSB-MP390352BPV
CSB-EP390352BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Leucine--tRNA ligase (leuS), partial CSB-YP390353BPV
CSB-EP390353BPV
CSB-BP390353BPV
CSB-MP390353BPV
CSB-EP390353BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S9 (rpsI) CSB-YP390354BPV
CSB-EP390354BPV
CSB-BP390354BPV
CSB-MP390354BPV
CSB-EP390354BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP390355BPV
CSB-EP390355BPV
CSB-BP390355BPV
CSB-MP390355BPV
CSB-EP390355BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Catalase-peroxidase 1 (katG1), partial CSB-YP390356BPV
CSB-EP390356BPV
CSB-BP390356BPV
CSB-MP390356BPV
CSB-EP390356BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP390357BPV
CSB-EP390357BPV
CSB-BP390357BPV
CSB-MP390357BPV
CSB-EP390357BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP390358BPV
CSB-EP390358BPV
CSB-BP390358BPV
CSB-MP390358BPV
CSB-EP390358BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Homogentisate 1,2-dioxygenase (hmgA) CSB-YP390359BPV
CSB-EP390359BPV
CSB-BP390359BPV
CSB-MP390359BPV
CSB-EP390359BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0301 protein Bcep1808_0798 (Bcep1808_0798) CSB-YP390360BPV
CSB-EP390360BPV
CSB-BP390360BPV
CSB-MP390360BPV
CSB-EP390360BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Aspartate carbamoyltransferase (pyrB) CSB-YP390361BPV
CSB-EP390361BPV
CSB-BP390361BPV
CSB-MP390361BPV
CSB-EP390361BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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