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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Shewanella loihica tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP389102STO
CSB-EP389102STO
CSB-BP389102STO
CSB-MP389102STO
CSB-EP389102STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glucosamine-6-phosphate deaminase (nagB) CSB-YP389103STO
CSB-EP389103STO
CSB-BP389103STO
CSB-MP389103STO
CSB-EP389103STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein slyX homolog (slyX) CSB-YP389104STO
CSB-EP389104STO
CSB-BP389104STO
CSB-MP389104STO
CSB-EP389104STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L27 (rpmA) CSB-YP389105STO
CSB-EP389105STO
CSB-BP389105STO
CSB-MP389105STO
CSB-EP389105STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S21 (rpsU) CSB-YP389106STO
CSB-EP389106STO
CSB-BP389106STO
CSB-MP389106STO
CSB-EP389106STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP389107STO
CSB-EP389107STO
CSB-BP389107STO
CSB-MP389107STO
CSB-EP389107STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica RNA pyrophosphohydrolase (rppH) CSB-YP389108STO
CSB-EP389108STO
CSB-BP389108STO
CSB-MP389108STO
CSB-EP389108STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Elongation factor 4 (lepA), partial CSB-YP389109STO
CSB-EP389109STO
CSB-BP389109STO
CSB-MP389109STO
CSB-EP389109STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S16 (rpsP) CSB-YP389110STO
CSB-EP389110STO
CSB-BP389110STO
CSB-MP389110STO
CSB-EP389110STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S20 (rpsT) CSB-YP389111STO
CSB-EP389111STO
CSB-BP389111STO
CSB-MP389111STO
CSB-EP389111STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Isoleucine--tRNA ligase (ileS), partial CSB-YP389112STO
CSB-EP389112STO
CSB-BP389112STO
CSB-MP389112STO
CSB-EP389112STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica UPF0301 protein Shew_1144 (Shew_1144) CSB-YP389113STO
CSB-EP389113STO
CSB-BP389113STO
CSB-MP389113STO
CSB-EP389113STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Enolase (eno) CSB-YP389114STO
CSB-EP389114STO
CSB-BP389114STO
CSB-MP389114STO
CSB-EP389114STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP389115STO
CSB-EP389115STO
CSB-BP389115STO
CSB-MP389115STO
CSB-EP389115STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 5'-nucleotidase surE (surE) CSB-YP389116STO
CSB-EP389116STO
CSB-BP389116STO
CSB-MP389116STO
CSB-EP389116STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein RecA (recA) CSB-YP389117STO
CSB-EP389117STO
CSB-BP389117STO
CSB-MP389117STO
CSB-EP389117STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Carbon storage regulator homolog (csrA) CSB-YP389118STO
CSB-EP389118STO
CSB-BP389118STO
CSB-MP389118STO
CSB-EP389118STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP389119STO
CSB-EP389119STO
CSB-BP389119STO
CSB-MP389119STO
CSB-EP389119STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Probable phosphatase Shew_1420 (Shew_1420) CSB-YP389120STO
CSB-EP389120STO
CSB-BP389120STO
CSB-MP389120STO
CSB-EP389120STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glutamate--tRNA ligase (gltX) CSB-YP389121STO
CSB-EP389121STO
CSB-BP389121STO
CSB-MP389121STO
CSB-EP389121STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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