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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Thymine dioxygenase JBP1 (JBP1), partial CSB-YP390102LOF
CSB-BP390102LOF
CSB-MP390102LOF
CSB-EP390102LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATPase ASNA1 homolog (LinJ11.0710, LinJ_11_0710) CSB-YP390103LOF
CSB-EP390103LOF
CSB-BP390103LOF
CSB-MP390103LOF
CSB-EP390103LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional helicase and thymine dioxygenase JBP2 (JBP2), partial CSB-YP390104LOF
CSB-EP390104LOF
CSB-BP390104LOF
CSB-MP390104LOF
CSB-EP390104LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional NAD (P)H-hydrate repair enzyme, partial CSB-YP390105LOF
CSB-EP390105LOF
CSB-BP390105LOF
CSB-MP390105LOF
CSB-EP390105LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ecotin-like protein 3 (LinJ15.0540, LinJ_15_0540) CSB-YP390106LOF
CSB-EP390106LOF
CSB-BP390106LOF
CSB-MP390106LOF
CSB-EP390106LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (LINJ_20_0980) CSB-YP390107LOF
CSB-EP390107LOF
CSB-BP390107LOF
CSB-MP390107LOF
CSB-EP390107LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S3a-1 (LinJ35.0500, LinJ_35_0400) CSB-YP390108LOF
CSB-EP390108LOF
CSB-BP390108LOF
CSB-MP390108LOF
CSB-EP390108LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C1orf226 homolog CSB-YP390109BO
CSB-EP390109BO
CSB-BP390109BO
CSB-MP390109BO
CSB-EP390109BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM115 (fam115), partial CSB-YP390110DIL
CSB-EP390110DIL
CSB-BP390110DIL
CSB-MP390110DIL
CSB-EP390110DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Radical S-adenosyl methionine domain-containing protein 1, mitochondrial (rsad1) CSB-YP390111DIL
CSB-EP390111DIL
CSB-BP390111DIL
CSB-MP390111DIL
CSB-EP390111DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Major facilitator superfamily domain-containing protein 2B (mfsd2b), partial CSB-YP390112XBF
CSB-EP390112XBF
CSB-BP390112XBF
CSB-MP390112XBF
CSB-EP390112XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis T-cell leukemia translocation-altered gene protein homolog (tcta), partial CSB-YP390113XBF1
CSB-EP390113XBF1
CSB-BP390113XBF1
CSB-MP390113XBF1
CSB-EP390113XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0534 protein C4orf43 homolog CSB-YP390114XBF
CSB-EP390114XBF
CSB-BP390114XBF
CSB-MP390114XBF
CSB-EP390114XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0532 protein C7orf60 homolog CSB-YP390115XBF
CSB-EP390115XBF
CSB-BP390115XBF
CSB-MP390115XBF
CSB-EP390115XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein NDNF (ndnf), partial CSB-YP390116XBF
CSB-EP390116XBF
CSB-BP390116XBF
CSB-MP390116XBF
CSB-EP390116XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Cyclin-dependent kinase 14 (cdk14) CSB-YP390117XBF
CSB-EP390117XBF
CSB-BP390117XBF
CSB-MP390117XBF
CSB-EP390117XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Serine--tRNA ligase (serS) CSB-YP390118GEH
CSB-EP390118GEH
CSB-BP390118GEH
CSB-MP390118GEH
CSB-EP390118GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Recombination protein RecR (recR) CSB-YP390119GEH
CSB-EP390119GEH
CSB-BP390119GEH
CSB-MP390119GEH
CSB-EP390119GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Bifunctional protein GlmU (glmU) CSB-YP390120GEH
CSB-EP390120GEH
CSB-BP390120GEH
CSB-MP390120GEH
CSB-EP390120GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Putative ATP:guanido phosphotransferase GTNG_0077 (GTNG_0077) CSB-YP390121GEH
CSB-EP390121GEH
CSB-BP390121GEH
CSB-MP390121GEH
CSB-EP390121GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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