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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotomaculum reducens Aspartate carbamoyltransferase (pyrB) CSB-YP390302DHX
CSB-EP390302DHX
CSB-BP390302DHX
CSB-MP390302DHX
CSB-EP390302DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UPF0173 metal-dependent hydrolase Dred_1740 (Dred_1740) CSB-YP390303DHX
CSB-EP390303DHX
CSB-BP390303DHX
CSB-MP390303DHX
CSB-EP390303DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP390304DHX
CSB-EP390304DHX
CSB-BP390304DHX
CSB-MP390304DHX
CSB-EP390304DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 5'-nucleotidase surE (surE) CSB-YP390305DHX
CSB-EP390305DHX
CSB-BP390305DHX
CSB-MP390305DHX
CSB-EP390305DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Protein tlp homolog (tlp) CSB-YP390306DHX
CSB-EP390306DHX
CSB-BP390306DHX
CSB-MP390306DHX
CSB-EP390306DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens D-alanine--D-alanine ligase (ddl) CSB-YP390307DHX
CSB-EP390307DHX
CSB-BP390307DHX
CSB-MP390307DHX
CSB-EP390307DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Putative competence-damage inducible protein (cinA) CSB-YP390308DHX
CSB-EP390308DHX
CSB-BP390308DHX
CSB-MP390308DHX
CSB-EP390308DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 30S ribosomal protein S15 (rpsO) CSB-YP390309DHX
CSB-EP390309DHX
CSB-BP390309DHX
CSB-MP390309DHX
CSB-EP390309DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 30S ribosomal protein S2 (rpsB) CSB-YP390310DHX
CSB-EP390310DHX
CSB-BP390310DHX
CSB-MP390310DHX
CSB-EP390310DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UPF0102 protein Dred_2035 (Dred_2035) CSB-YP390311DHX
CSB-EP390311DHX
CSB-BP390311DHX
CSB-MP390311DHX
CSB-EP390311DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP390312DHX
CSB-EP390312DHX
CSB-BP390312DHX
CSB-MP390312DHX
CSB-EP390312DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP390313DHX
CSB-EP390313DHX
CSB-BP390313DHX
CSB-MP390313DHX
CSB-EP390313DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP390314DHX
CSB-EP390314DHX
CSB-BP390314DHX
CSB-MP390314DHX
CSB-EP390314DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP390315DHX
CSB-EP390315DHX
CSB-BP390315DHX
CSB-MP390315DHX
CSB-EP390315DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP390316DHX
CSB-EP390316DHX
CSB-BP390316DHX
CSB-MP390316DHX
CSB-EP390316DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Ribonuclease H (rnhA) CSB-YP390317DHX
CSB-EP390317DHX
CSB-BP390317DHX
CSB-MP390317DHX
CSB-EP390317DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP390318DHX
CSB-EP390318DHX
CSB-BP390318DHX
CSB-MP390318DHX
CSB-EP390318DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Ribosomal protein L11 methyltransferase (prmA) CSB-YP390319DHX
CSB-EP390319DHX
CSB-BP390319DHX
CSB-MP390319DHX
CSB-EP390319DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Elongation factor 4 (lepA), partial CSB-YP390320DHX
CSB-EP390320DHX
CSB-BP390320DHX
CSB-MP390320DHX
CSB-EP390320DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Leucine--tRNA ligase (leuS), partial CSB-YP390321DHX
CSB-EP390321DHX
CSB-BP390321DHX
CSB-MP390321DHX
CSB-EP390321DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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