Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L20 (rplT) CSB-YP389282STB
CSB-EP389282STB
CSB-BP389282STB
CSB-MP389282STB
CSB-EP389282STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0261 protein SACE_5696 (SACE_5696) CSB-YP389283STB
CSB-EP389283STB
CSB-BP389283STB
CSB-MP389283STB
CSB-EP389283STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP389284STB
CSB-EP389284STB
CSB-BP389284STB
CSB-MP389284STB
CSB-EP389284STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP389285STB
CSB-EP389285STB
CSB-BP389285STB
CSB-MP389285STB
CSB-EP389285STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP389286STB
CSB-EP389286STB
CSB-BP389286STB
CSB-MP389286STB
CSB-EP389286STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP389287STB
CSB-EP389287STB
CSB-BP389287STB
CSB-MP389287STB
CSB-EP389287STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S15 (rpsO) CSB-YP389288STB
CSB-EP389288STB
CSB-BP389288STB
CSB-MP389288STB
CSB-EP389288STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0102 protein SACE_6045 (SACE_6045) CSB-YP389289STB
CSB-EP389289STB
CSB-BP389289STB
CSB-MP389289STB
CSB-EP389289STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Ribosome maturation factor RimM (rimM) CSB-YP389290STB
CSB-EP389290STB
CSB-BP389290STB
CSB-MP389290STB
CSB-EP389290STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP389291STB
CSB-EP389291STB
CSB-BP389291STB
CSB-MP389291STB
CSB-EP389291STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Ribonuclease H (rnhA) CSB-YP389292STB
CSB-EP389292STB
CSB-BP389292STB
CSB-MP389292STB
CSB-EP389292STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Glycosyl hydrolase family 109 protein (SACE_6314) CSB-YP389293STB
CSB-EP389293STB
CSB-BP389293STB
CSB-MP389293STB
CSB-EP389293STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Protein translocase subunit SecA (secA), partial CSB-YP389294STB
CSB-EP389294STB
CSB-BP389294STB
CSB-MP389294STB
CSB-EP389294STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S9 (rpsI) CSB-YP389295STB
CSB-EP389295STB
CSB-BP389295STB
CSB-MP389295STB
CSB-EP389295STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP389296STB
CSB-EP389296STB
CSB-BP389296STB
CSB-MP389296STB
CSB-EP389296STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S13 (rpsM) CSB-YP389297STB
CSB-EP389297STB
CSB-BP389297STB
CSB-MP389297STB
CSB-EP389297STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L30 (rpmD) CSB-YP389298STB
CSB-EP389298STB
CSB-BP389298STB
CSB-MP389298STB
CSB-EP389298STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L6 (rplF) CSB-YP389299STB
CSB-EP389299STB
CSB-BP389299STB
CSB-MP389299STB
CSB-EP389299STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L14 (rplN) CSB-YP389300STB
CSB-EP389300STB
CSB-BP389300STB
CSB-MP389300STB
CSB-EP389300STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L23 (rplW) CSB-YP389301STB
CSB-EP389301STB
CSB-BP389301STB
CSB-MP389301STB
CSB-EP389301STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>