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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP390962MOK
CSB-EP390962MOK
CSB-BP390962MOK
CSB-MP390962MOK
CSB-EP390962MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Urease subunit gamma (ureA) CSB-YP390963MOK
CSB-EP390963MOK
CSB-BP390963MOK
CSB-MP390963MOK
CSB-EP390963MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Proteasome subunit alpha (prcA) CSB-YP390964MOK
CSB-EP390964MOK
CSB-BP390964MOK
CSB-MP390964MOK
CSB-EP390964MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum R2-like ligand binding oxidase (Mflv_3061) CSB-YP390965MOK
CSB-EP390965MOK
CSB-BP390965MOK
CSB-MP390965MOK
CSB-EP390965MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Dihydroorotate dehydrogenase (quinone) CSB-YP390966MOK
CSB-EP390966MOK
CSB-BP390966MOK
CSB-MP390966MOK
CSB-EP390966MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP390967MOK
CSB-EP390967MOK
CSB-BP390967MOK
CSB-MP390967MOK
CSB-EP390967MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Anthranilate phosphoribosyltransferase (trpD) CSB-YP390968MOK
CSB-EP390968MOK
CSB-BP390968MOK
CSB-MP390968MOK
CSB-EP390968MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Aspartate--tRNA ligase (aspS), partial CSB-YP390969MOK
CSB-EP390969MOK
CSB-BP390969MOK
CSB-MP390969MOK
CSB-EP390969MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP390970MOK
CSB-EP390970MOK
CSB-BP390970MOK
CSB-MP390970MOK
CSB-EP390970MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable malate:quinone oxidoreductase (mqo), partial CSB-YP390971MOK
CSB-EP390971MOK
CSB-BP390971MOK
CSB-MP390971MOK
CSB-EP390971MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S15 (rpsO) CSB-YP390972MOK
CSB-EP390972MOK
CSB-BP390972MOK
CSB-MP390972MOK
CSB-EP390972MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Dihydrodipicolinate reductase (dapB) CSB-YP390973MOK
CSB-EP390973MOK
CSB-BP390973MOK
CSB-MP390973MOK
CSB-EP390973MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Thymidylate synthase thyX (thyX) CSB-YP390974MOK
CSB-EP390974MOK
CSB-BP390974MOK
CSB-MP390974MOK
CSB-EP390974MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable transcriptional regulatory protein Mflv_3828 (Mflv_3828) CSB-YP390975MOK
CSB-EP390975MOK
CSB-BP390975MOK
CSB-MP390975MOK
CSB-EP390975MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP390976MOK
CSB-EP390976MOK
CSB-BP390976MOK
CSB-MP390976MOK
CSB-EP390976MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Peptide chain release factor 2 (prfB) CSB-YP390977MOK
CSB-EP390977MOK
CSB-BP390977MOK
CSB-MP390977MOK
CSB-EP390977MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable DNA ligase (lig), partial CSB-YP390978MOK
CSB-EP390978MOK
CSB-BP390978MOK
CSB-MP390978MOK
CSB-EP390978MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP390979MOK
CSB-EP390979MOK
CSB-BP390979MOK
CSB-MP390979MOK
CSB-EP390979MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glutamate--tRNA ligase (gltX) CSB-YP390980MOK
CSB-EP390980MOK
CSB-BP390980MOK
CSB-MP390980MOK
CSB-EP390980MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum D-alanine--D-alanine ligase (ddl) CSB-YP390981MOK
CSB-EP390981MOK
CSB-BP390981MOK
CSB-MP390981MOK
CSB-EP390981MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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