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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Polynucleobacter sp. 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP390922PYU
CSB-EP390922PYU
CSB-BP390922PYU
CSB-MP390922PYU
CSB-EP390922PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP390923PYU
CSB-EP390923PYU
CSB-BP390923PYU
CSB-MP390923PYU
CSB-EP390923PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP390924PYU
CSB-EP390924PYU
CSB-BP390924PYU
CSB-MP390924PYU
CSB-EP390924PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP390925PYU
CSB-EP390925PYU
CSB-BP390925PYU
CSB-MP390925PYU
CSB-EP390925PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP390926PYU
CSB-EP390926PYU
CSB-BP390926PYU
CSB-MP390926PYU
CSB-EP390926PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP390927PYU
CSB-EP390927PYU
CSB-BP390927PYU
CSB-MP390927PYU
CSB-EP390927PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP390928PYU
CSB-EP390928PYU
CSB-BP390928PYU
CSB-MP390928PYU
CSB-EP390928PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP390929PYU
CSB-EP390929PYU
CSB-BP390929PYU
CSB-MP390929PYU
CSB-EP390929PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Acetylglutamate kinase (argB) CSB-YP390930PYU
CSB-EP390930PYU
CSB-BP390930PYU
CSB-MP390930PYU
CSB-EP390930PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Catalase-peroxidase (katG), partial CSB-YP390931PYU
CSB-EP390931PYU
CSB-BP390931PYU
CSB-MP390931PYU
CSB-EP390931PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. tRNA modification GTPase MnmE (mnmE) CSB-YP390932PYU
CSB-EP390932PYU
CSB-BP390932PYU
CSB-MP390932PYU
CSB-EP390932PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Trans-aconitate 2-methyltransferase (tam) CSB-YP390933MOK
CSB-EP390933MOK
CSB-BP390933MOK
CSB-MP390933MOK
CSB-EP390933MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Na (+)/H (+) antiporter nhaA 1, partial CSB-YP390934MOK
CSB-EP390934MOK
CSB-BP390934MOK
CSB-MP390934MOK
CSB-EP390934MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Acetate kinase (ackA) CSB-YP390935MOK
CSB-EP390935MOK
CSB-BP390935MOK
CSB-MP390935MOK
CSB-EP390935MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L33 2 (rpmG2) CSB-YP390936MOK
CSB-EP390936MOK
CSB-BP390936MOK
CSB-MP390936MOK
CSB-EP390936MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L11 (rplK) CSB-YP390937MOK
CSB-EP390937MOK
CSB-BP390937MOK
CSB-MP390937MOK
CSB-EP390937MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L7/L12 (rplL) CSB-YP390938MOK
CSB-EP390938MOK
CSB-BP390938MOK
CSB-MP390938MOK
CSB-EP390938MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L23 (rplW) CSB-YP390939MOK
CSB-EP390939MOK
CSB-BP390939MOK
CSB-MP390939MOK
CSB-EP390939MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable rRNA maturation factor (Mflv_2707) CSB-YP390940MOK
CSB-EP390940MOK
CSB-BP390940MOK
CSB-MP390940MOK
CSB-EP390940MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribonuclease Z (rnz) CSB-YP390941MOK
CSB-EP390941MOK
CSB-BP390941MOK
CSB-MP390941MOK
CSB-EP390941MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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