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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia vietnamiensis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP390402BPV
CSB-EP390402BPV
CSB-BP390402BPV
CSB-MP390402BPV
CSB-EP390402BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L25 (rplY) CSB-YP390403BPV
CSB-EP390403BPV
CSB-BP390403BPV
CSB-MP390403BPV
CSB-EP390403BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Protein-export protein secB (secB) CSB-YP390404BPV
CSB-EP390404BPV
CSB-BP390404BPV
CSB-MP390404BPV
CSB-EP390404BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Octanoyltransferase (lipB) CSB-YP390405BPV
CSB-EP390405BPV
CSB-BP390405BPV
CSB-MP390405BPV
CSB-EP390405BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Arginine--tRNA ligase (argS), partial CSB-YP390406BPV
CSB-EP390406BPV
CSB-BP390406BPV
CSB-MP390406BPV
CSB-EP390406BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Biotin synthase (bioB) CSB-YP390407BPV
CSB-EP390407BPV
CSB-BP390407BPV
CSB-MP390407BPV
CSB-EP390407BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Flagellar hook-basal body complex protein FliE (fliE) CSB-YP390408BPV
CSB-EP390408BPV
CSB-BP390408BPV
CSB-MP390408BPV
CSB-EP390408BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Diaminopimelate epimerase (dapF) CSB-YP390409BPV
CSB-EP390409BPV
CSB-BP390409BPV
CSB-MP390409BPV
CSB-EP390409BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP390410BPV
CSB-EP390410BPV
CSB-BP390410BPV
CSB-MP390410BPV
CSB-EP390410BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis tRNA modification GTPase MnmE (mnmE) CSB-YP390411BPV
CSB-EP390411BPV
CSB-BP390411BPV
CSB-MP390411BPV
CSB-EP390411BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Putative membrane protein insertion efficiency factor (Bcep1808_3311) CSB-YP390412BPV
CSB-EP390412BPV
CSB-BP390412BPV
CSB-MP390412BPV
CSB-EP390412BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis HTH-type transcriptional regulator BetI (betI) CSB-YP390413BPV
CSB-EP390413BPV
CSB-BP390413BPV
CSB-MP390413BPV
CSB-EP390413BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Acylphosphatase (acyP) CSB-YP390414BPV
CSB-EP390414BPV
CSB-BP390414BPV
CSB-MP390414BPV
CSB-EP390414BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Homoserine kinase (thrB) CSB-YP390415BPV
CSB-EP390415BPV
CSB-BP390415BPV
CSB-MP390415BPV
CSB-EP390415BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Lipase chaperone (lifO), partial CSB-YP390416BPV1
CSB-EP390416BPV1
CSB-BP390416BPV1
CSB-MP390416BPV1
CSB-EP390416BPV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 60 kDa chaperonin 2 (groL2), partial CSB-YP390417BPV
CSB-EP390417BPV
CSB-BP390417BPV
CSB-MP390417BPV
CSB-EP390417BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (mhpC) CSB-YP390418BPV
CSB-EP390418BPV
CSB-BP390418BPV
CSB-MP390418BPV
CSB-EP390418BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase 1, partial CSB-YP390419BPV
CSB-EP390419BPV
CSB-BP390419BPV
CSB-MP390419BPV
CSB-EP390419BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP390420BPV
CSB-EP390420BPV
CSB-BP390420BPV
CSB-MP390420BPV
CSB-EP390420BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Putative iron-sulfur cluster insertion protein ErpA 2 (erpA2) CSB-YP390421BPV
CSB-EP390421BPV
CSB-BP390421BPV
CSB-MP390421BPV
CSB-EP390421BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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