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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas salmonicida Ribosome maturation factor RimP (rimP) CSB-YP390802AUI
CSB-EP390802AUI
CSB-BP390802AUI
CSB-MP390802AUI
CSB-EP390802AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP390803AUI
CSB-EP390803AUI
CSB-BP390803AUI
CSB-MP390803AUI
CSB-EP390803AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP390804AUI
CSB-EP390804AUI
CSB-BP390804AUI
CSB-MP390804AUI
CSB-EP390804AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable rRNA maturation factor (ASA_1072) CSB-YP390805AUI
CSB-EP390805AUI
CSB-BP390805AUI
CSB-MP390805AUI
CSB-EP390805AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Elongation factor P (efp) CSB-YP390806AUI
CSB-EP390806AUI
CSB-BP390806AUI
CSB-MP390806AUI
CSB-EP390806AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Shikimate kinase (aroK) CSB-YP390807AUI
CSB-EP390807AUI
CSB-BP390807AUI
CSB-MP390807AUI
CSB-EP390807AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP390808AUI
CSB-EP390808AUI
CSB-BP390808AUI
CSB-MP390808AUI
CSB-EP390808AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Putative Holliday junction resolvase (ASA_1194) CSB-YP390809AUI
CSB-EP390809AUI
CSB-BP390809AUI
CSB-MP390809AUI
CSB-EP390809AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP390810AUI
CSB-EP390810AUI
CSB-BP390810AUI
CSB-MP390810AUI
CSB-EP390810AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable phosphatase ASA_1316 (ASA_1316) CSB-YP390811AUI
CSB-EP390811AUI
CSB-BP390811AUI
CSB-MP390811AUI
CSB-EP390811AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP390812AUI
CSB-EP390812AUI
CSB-BP390812AUI
CSB-MP390812AUI
CSB-EP390812AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Tryptophan synthase beta chain (trpB) CSB-YP390813AUI
CSB-EP390813AUI
CSB-BP390813AUI
CSB-MP390813AUI
CSB-EP390813AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Uracil phosphoribosyltransferase (upp) CSB-YP390814AUI
CSB-EP390814AUI
CSB-BP390814AUI
CSB-MP390814AUI
CSB-EP390814AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP390815AUI1
CSB-EP390815AUI1
CSB-BP390815AUI1
CSB-MP390815AUI1
CSB-EP390815AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP390816AUI
CSB-EP390816AUI
CSB-BP390816AUI
CSB-MP390816AUI
CSB-EP390816AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Integration host factor subunit beta (ihfB) CSB-YP390817AUI
CSB-EP390817AUI
CSB-BP390817AUI
CSB-MP390817AUI
CSB-EP390817AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable potassium transport system protein kup (kup), partial CSB-YP390818AUI
CSB-EP390818AUI
CSB-BP390818AUI
CSB-MP390818AUI
CSB-EP390818AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Trigger factor (tig) CSB-YP390819AUI
CSB-EP390819AUI
CSB-BP390819AUI
CSB-MP390819AUI
CSB-EP390819AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Chorismate synthase (aroC) CSB-YP390820AUI
CSB-EP390820AUI
CSB-BP390820AUI
CSB-MP390820AUI
CSB-EP390820AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP390821AUI
CSB-EP390821AUI
CSB-BP390821AUI
CSB-MP390821AUI
CSB-EP390821AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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