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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis 30S ribosomal protein S11 (rpsK) CSB-YP391002YAL
CSB-EP391002YAL
CSB-BP391002YAL
CSB-MP391002YAL
CSB-EP391002YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP391003YAL
CSB-EP391003YAL
CSB-BP391003YAL
CSB-MP391003YAL
CSB-EP391003YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP391004YAL
CSB-EP391004YAL
CSB-BP391004YAL
CSB-MP391004YAL
CSB-EP391004YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP391005YAL1
CSB-EP391005YAL1
CSB-BP391005YAL1
CSB-MP391005YAL1
CSB-EP391005YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0307 protein YPDSF_0298 (YPDSF_0298) CSB-YP391006YAL
CSB-EP391006YAL
CSB-BP391006YAL
CSB-MP391006YAL
CSB-EP391006YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0042 nucleotide-binding protein YPDSF_0311 (YPDSF_0311) CSB-YP391007YAL
CSB-EP391007YAL
CSB-BP391007YAL
CSB-MP391007YAL
CSB-EP391007YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S9 (rpsI) CSB-YP391008YAL
CSB-EP391008YAL
CSB-BP391008YAL
CSB-MP391008YAL
CSB-EP391008YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0102 protein YPDSF_0348 (YPDSF_0348) CSB-YP391009YAL
CSB-EP391009YAL
CSB-BP391009YAL
CSB-MP391009YAL
CSB-EP391009YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Serine/threonine transporter sstT (sstT), partial CSB-YP391010YAL
CSB-EP391010YAL
CSB-BP391010YAL
CSB-MP391010YAL
CSB-EP391010YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP391011YAL
CSB-EP391011YAL
CSB-BP391011YAL
CSB-MP391011YAL
CSB-EP391011YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Bifunctional protein HldE (hldE) CSB-YP391012YAL
CSB-EP391012YAL
CSB-BP391012YAL
CSB-MP391012YAL
CSB-EP391012YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0235 protein YPDSF_0571 (YPDSF_0571) CSB-YP391013YAL
CSB-EP391013YAL
CSB-BP391013YAL
CSB-MP391013YAL
CSB-EP391013YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphoglycerate kinase (pgk) CSB-YP391014YAL
CSB-EP391014YAL
CSB-BP391014YAL
CSB-MP391014YAL
CSB-EP391014YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Maltoporin 2 (lamB2) CSB-YP391015YAL
CSB-EP391015YAL
CSB-BP391015YAL
CSB-MP391015YAL
CSB-EP391015YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 50S ribosomal protein L20 (rplT) CSB-YP391016YAL
CSB-EP391016YAL
CSB-BP391016YAL
CSB-MP391016YAL
CSB-EP391016YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP391017YAL
CSB-EP391017YAL
CSB-BP391017YAL
CSB-MP391017YAL
CSB-EP391017YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Putative phosphotransferase YPDSF_0739 (YPDSF_0739) CSB-YP391018YAL
CSB-EP391018YAL
CSB-BP391018YAL
CSB-MP391018YAL
CSB-EP391018YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis HTH-type transcriptional repressor PurR (purR) CSB-YP391019YAL
CSB-EP391019YAL
CSB-BP391019YAL
CSB-MP391019YAL
CSB-EP391019YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Tyrosine--tRNA ligase (tyrS) CSB-YP391020YAL
CSB-EP391020YAL
CSB-BP391020YAL
CSB-MP391020YAL
CSB-EP391020YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0312 protein YPDSF_0829 (YPDSF_0829) CSB-YP391021YAL
CSB-EP391021YAL
CSB-BP391021YAL
CSB-MP391021YAL
CSB-EP391021YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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