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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP391362ILO
CSB-BP391362ILO
CSB-MP391362ILO
CSB-EP391362ILO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Protein PB1-F2 (PB1-F2) CSB-YP391363ILO
CSB-EP391363ILO
CSB-BP391363ILO
CSB-MP391363ILO
CSB-EP391363ILO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bufo bufo gargarizans UPF0451 protein C17orf61 homolog, partial CSB-YP391364BPL
CSB-EP391364BPL
CSB-BP391364BPL
CSB-MP391364BPL
CSB-EP391364BPL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat C-type lectin domain family 2 member D2 (Clec2d2), partial CSB-YP391365RA
CSB-EP391365RA
CSB-BP391365RA
CSB-MP391365RA
CSB-EP391365RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens ascovirus 3e Putative ATP-dependent RNA helicase (ORF15), partial CSB-YP391366HSI
CSB-EP391366HSI
CSB-BP391366HSI
CSB-MP391366HSI
CSB-EP391366HSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens ascovirus 3e Major capsid protein (MCP) CSB-YP391367HSI
CSB-EP391367HSI
CSB-BP391367HSI
CSB-MP391367HSI
CSB-EP391367HSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens ascovirus 3e Uncharacterized protein ORF58 (ORF58), partial CSB-YP391368HSI1
CSB-EP391368HSI1
CSB-BP391368HSI1
CSB-MP391368HSI1
CSB-EP391368HSI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat PIN2/TERF1-interacting telomerase inhibitor 1 (Pinx1) CSB-YP391369RA
CSB-EP391369RA
CSB-BP391369RA
CSB-MP391369RA
CSB-EP391369RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Centrosomal protein KIAA1731 homolog, partial CSB-YP391370RA
CSB-EP391370RA
CSB-BP391370RA
CSB-MP391370RA
CSB-EP391370RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice gall dwarf virus RNA-directed RNA polymerase P1, partial CSB-YP391371RMJ
CSB-EP391371RMJ
CSB-BP391371RMJ
CSB-MP391371RMJ
CSB-EP391371RMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0213 protein in potE 3'region CSB-YP391372STI
CSB-EP391372STI
CSB-BP391372STI
CSB-MP391372STI
CSB-EP391372STI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0233 membrane protein cgR_0053 (cgR_0053), partial CSB-YP391373CXG1
CSB-EP391373CXG1
CSB-BP391373CXG1
CSB-MP391373CXG1
CSB-EP391373CXG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Urease accessory protein UreF (ureF) CSB-YP391374CXG
CSB-EP391374CXG
CSB-BP391374CXG
CSB-MP391374CXG
CSB-EP391374CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Pantothenate synthetase (panC) CSB-YP391375CXG
CSB-EP391375CXG
CSB-BP391375CXG
CSB-MP391375CXG
CSB-EP391375CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP391376CXG
CSB-EP391376CXG
CSB-BP391376CXG
CSB-MP391376CXG
CSB-EP391376CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Recombination protein RecR (recR) CSB-YP391377CXG
CSB-EP391377CXG
CSB-BP391377CXG
CSB-MP391377CXG
CSB-EP391377CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP391378CXG
CSB-EP391378CXG
CSB-BP391378CXG
CSB-MP391378CXG
CSB-EP391378CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 3-dehydroquinate dehydratase (aroQ) CSB-YP391379CXG
CSB-EP391379CXG
CSB-BP391379CXG
CSB-MP391379CXG
CSB-EP391379CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Demethylmenaquinone methyltransferase (ubiE) CSB-YP391380CXG
CSB-EP391380CXG
CSB-BP391380CXG
CSB-MP391380CXG
CSB-EP391380CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L10 (rplJ) CSB-YP391381CXG
CSB-EP391381CXG
CSB-BP391381CXG
CSB-MP391381CXG
CSB-EP391381CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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