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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacillus thermodenitrificans Cobyric acid synthase (cobQ) CSB-YP391102GEH
CSB-EP391102GEH
CSB-BP391102GEH
CSB-MP391102GEH
CSB-EP391102GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP391103GEH
CSB-EP391103GEH
CSB-BP391103GEH
CSB-MP391103GEH
CSB-EP391103GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP391104GEH
CSB-EP391104GEH
CSB-BP391104GEH
CSB-MP391104GEH
CSB-EP391104GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 3-dehydroquinate dehydratase (aroD) CSB-YP391105GEH
CSB-EP391105GEH
CSB-BP391105GEH
CSB-MP391105GEH
CSB-EP391105GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Asparagine--tRNA ligase (asnS) CSB-YP391106GEH
CSB-EP391106GEH
CSB-BP391106GEH
CSB-MP391106GEH
CSB-EP391106GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Histidinol-phosphate aminotransferase (hisC) CSB-YP391107GEH
CSB-EP391107GEH
CSB-BP391107GEH
CSB-MP391107GEH
CSB-EP391107GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Tryptophan synthase alpha chain (trpA) CSB-YP391108GEH
CSB-EP391108GEH
CSB-BP391108GEH
CSB-MP391108GEH
CSB-EP391108GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Anthranilate phosphoribosyltransferase (trpD) CSB-YP391109GEH
CSB-EP391109GEH
CSB-BP391109GEH
CSB-MP391109GEH
CSB-EP391109GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP391110GEH
CSB-EP391110GEH
CSB-BP391110GEH
CSB-MP391110GEH
CSB-EP391110GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Thiol-disulfide oxidoreductase resA (resA), partial CSB-YP391111GEH1
CSB-EP391111GEH1
CSB-BP391111GEH1
CSB-MP391111GEH1
CSB-EP391111GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Segregation and condensation protein B (scpB) CSB-YP391112GEH
CSB-EP391112GEH
CSB-BP391112GEH
CSB-MP391112GEH
CSB-EP391112GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP391113GEH
CSB-EP391113GEH
CSB-BP391113GEH
CSB-MP391113GEH
CSB-EP391113GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP391114GEH
CSB-EP391114GEH
CSB-BP391114GEH
CSB-MP391114GEH
CSB-EP391114GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Chaperone protein DnaJ (dnaJ) CSB-YP391115GEH
CSB-EP391115GEH
CSB-BP391115GEH
CSB-MP391115GEH
CSB-EP391115GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP391116GEH
CSB-EP391116GEH
CSB-BP391116GEH
CSB-MP391116GEH
CSB-EP391116GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP391117GEH
CSB-EP391117GEH
CSB-BP391117GEH
CSB-MP391117GEH
CSB-EP391117GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP391118GEH
CSB-EP391118GEH
CSB-BP391118GEH
CSB-MP391118GEH
CSB-EP391118GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Quinolinate synthase A (nadA) CSB-YP391119GEH
CSB-EP391119GEH
CSB-BP391119GEH
CSB-MP391119GEH
CSB-EP391119GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L21 (rplU) CSB-YP391120GEH
CSB-EP391120GEH
CSB-BP391120GEH
CSB-MP391120GEH
CSB-EP391120GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Probable GTP-binding protein EngB (engB) CSB-YP391121GEH
CSB-EP391121GEH
CSB-BP391121GEH
CSB-MP391121GEH
CSB-EP391121GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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