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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L7/L12 (rplL) CSB-YP391062GEH
CSB-EP391062GEH
CSB-BP391062GEH
CSB-MP391062GEH
CSB-EP391062GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S12 (rpsL) CSB-YP391063GEH
CSB-EP391063GEH
CSB-BP391063GEH
CSB-MP391063GEH
CSB-EP391063GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L4 (rplD) CSB-YP391064GEH
CSB-EP391064GEH
CSB-BP391064GEH
CSB-MP391064GEH
CSB-EP391064GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S3 (rpsC) CSB-YP391065GEH
CSB-EP391065GEH
CSB-BP391065GEH
CSB-MP391065GEH
CSB-EP391065GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S8 (rpsH) CSB-YP391066GEH
CSB-EP391066GEH
CSB-BP391066GEH
CSB-MP391066GEH
CSB-EP391066GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Translation initiation factor IF-1 (infA) CSB-YP391067GEH
CSB-EP391067GEH
CSB-BP391067GEH
CSB-MP391067GEH
CSB-EP391067GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP391068GEH
CSB-EP391068GEH
CSB-BP391068GEH
CSB-MP391068GEH
CSB-EP391068GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S9 (rpsI) CSB-YP391069GEH
CSB-EP391069GEH
CSB-BP391069GEH
CSB-MP391069GEH
CSB-EP391069GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP391070GEH
CSB-EP391070GEH
CSB-BP391070GEH
CSB-MP391070GEH
CSB-EP391070GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP391071GEH
CSB-EP391071GEH
CSB-BP391071GEH
CSB-MP391071GEH
CSB-EP391071GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 10 kDa chaperonin (groS) CSB-YP391072GEH
CSB-EP391072GEH
CSB-BP391072GEH
CSB-MP391072GEH
CSB-EP391072GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP391073GEH
CSB-EP391073GEH
CSB-BP391073GEH
CSB-MP391073GEH
CSB-EP391073GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP391074GEH
CSB-EP391074GEH
CSB-BP391074GEH
CSB-MP391074GEH
CSB-EP391074GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Acylphosphatase (acyP) CSB-YP391075GEH
CSB-EP391075GEH
CSB-BP391075GEH
CSB-MP391075GEH
CSB-EP391075GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0374 protein GTNG_0473 (GTNG_0473) CSB-YP391076GEH
CSB-EP391076GEH
CSB-BP391076GEH
CSB-MP391076GEH
CSB-EP391076GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0295 protein GTNG_0491 (GTNG_0491), partial CSB-YP391077GEH1
CSB-EP391077GEH1
CSB-BP391077GEH1
CSB-MP391077GEH1
CSB-EP391077GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0342 protein GTNG_0551 (GTNG_0551) CSB-YP391078GEH
CSB-EP391078GEH
CSB-BP391078GEH
CSB-MP391078GEH
CSB-EP391078GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0344 protein GTNG_0604 (GTNG_0604), partial CSB-YP391079GEH1
CSB-EP391079GEH1
CSB-BP391079GEH1
CSB-MP391079GEH1
CSB-EP391079GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP391080GEH
CSB-EP391080GEH
CSB-BP391080GEH
CSB-MP391080GEH
CSB-EP391080GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0477 protein GTNG_0743 (GTNG_0743) CSB-YP391081GEH
CSB-EP391081GEH
CSB-BP391081GEH
CSB-MP391081GEH
CSB-EP391081GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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