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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium gilvum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP391902MOK
CSB-EP391902MOK
CSB-BP391902MOK
CSB-MP391902MOK
CSB-EP391902MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Catalase-peroxidase (katG), partial CSB-YP391903MOK
CSB-EP391903MOK
CSB-BP391903MOK
CSB-MP391903MOK
CSB-EP391903MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Proteasome subunit beta (prcB) CSB-YP391904MOK
CSB-EP391904MOK
CSB-BP391904MOK
CSB-MP391904MOK
CSB-EP391904MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP phosphoribosyltransferase (hisG) CSB-YP391905MOK
CSB-EP391905MOK
CSB-BP391905MOK
CSB-MP391905MOK
CSB-EP391905MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Cell division protein SepF (sepF) CSB-YP391906MOK
CSB-EP391906MOK
CSB-BP391906MOK
CSB-MP391906MOK
CSB-EP391906MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Cobalamin synthase (cobS), partial CSB-YP391907MOK1
CSB-EP391907MOK1
CSB-BP391907MOK1
CSB-MP391907MOK1
CSB-EP391907MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP391908MOK
CSB-EP391908MOK
CSB-BP391908MOK
CSB-MP391908MOK
CSB-EP391908MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Alanine--tRNA ligase (alaS), partial CSB-YP391909MOK
CSB-EP391909MOK
CSB-BP391909MOK
CSB-MP391909MOK
CSB-EP391909MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3-dehydroquinate synthase (aroB) CSB-YP391910MOK
CSB-EP391910MOK
CSB-BP391910MOK
CSB-MP391910MOK
CSB-EP391910MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP391911MOK
CSB-EP391911MOK
CSB-BP391911MOK
CSB-MP391911MOK
CSB-EP391911MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP391912MOK
CSB-EP391912MOK
CSB-BP391912MOK
CSB-MP391912MOK
CSB-EP391912MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoglycerate kinase (pgk) CSB-YP391913MOK
CSB-EP391913MOK
CSB-BP391913MOK
CSB-MP391913MOK
CSB-EP391913MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Putative sporulation transcription regulator WhiA (whiA) CSB-YP391914MOK
CSB-EP391914MOK
CSB-BP391914MOK
CSB-MP391914MOK
CSB-EP391914MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S2 (rpsB) CSB-YP391915MOK
CSB-EP391915MOK
CSB-BP391915MOK
CSB-MP391915MOK
CSB-EP391915MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribosome-binding factor A (rbfA) CSB-YP391916MOK
CSB-EP391916MOK
CSB-BP391916MOK
CSB-MP391916MOK
CSB-EP391916MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP391917MOK
CSB-EP391917MOK
CSB-BP391917MOK
CSB-MP391917MOK
CSB-EP391917MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Dihydrodipicolinate synthase (dapA) CSB-YP391918MOK
CSB-EP391918MOK
CSB-BP391918MOK
CSB-MP391918MOK
CSB-EP391918MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP391919MOK
CSB-EP391919MOK
CSB-BP391919MOK
CSB-MP391919MOK
CSB-EP391919MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum LexA repressor (lexA) CSB-YP391920MOK
CSB-EP391920MOK
CSB-BP391920MOK
CSB-MP391920MOK
CSB-EP391920MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP391921MOK
CSB-EP391921MOK
CSB-BP391921MOK
CSB-MP391921MOK
CSB-EP391921MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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