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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thymine dioxygenase JBP1 (JBP1), partial CSB-YP391982LOC
CSB-EP391982LOC
CSB-BP391982LOC
CSB-MP391982LOC
CSB-EP391982LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional NAD (P)H-hydrate repair enzyme, partial CSB-YP391983LOC
CSB-EP391983LOC
CSB-BP391983LOC
CSB-MP391983LOC
CSB-EP391983LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ecotin-like protein 3 (LbrM15_V2.0550, LbrM_15_0550) CSB-YP391984LOC
CSB-EP391984LOC
CSB-BP391984LOC
CSB-MP391984LOC
CSB-EP391984LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoyl synthase, mitochondrial (LbrM19_V2.0670, LbrM_19_0670), partial CSB-YP391985LOC
CSB-EP391985LOC
CSB-BP391985LOC
CSB-MP391985LOC
CSB-EP391985LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts, mitochondrial (LbrM29_V2.0730, LbrM_29_0730), partial CSB-YP391986LOC
CSB-EP391986LOC
CSB-BP391986LOC
CSB-MP391986LOC
CSB-EP391986LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (LinJ09.1490, LinJ_09_1500), partial CSB-YP391987LOF
CSB-EP391987LOF
CSB-BP391987LOF
CSB-MP391987LOF
CSB-EP391987LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable citrate synthase, mitochondrial (LinJ18.0690, LinJ_18_0690), partial CSB-YP391988LOF
CSB-EP391988LOF
CSB-BP391988LOF
CSB-MP391988LOF
CSB-EP391988LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein SEY1 homolog (LinJ32.0740, LinJ_32_0380), partial CSB-YP391989LOF
CSB-EP391989LOF
CSB-BP391989LOF
CSB-MP391989LOF
CSB-EP391989LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S3a-2 (LinJ35.0510, LinJ_35_0410) CSB-YP391990LOF
CSB-EP391990LOF
CSB-BP391990LOF
CSB-MP391990LOF
CSB-EP391990LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine VEGF co-regulated chemokine 1 (CXCL17) CSB-YP391991BO
CSB-EP391991BO
CSB-BP391991BO
CSB-MP391991BO
CSB-EP391991BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitochondrial dynamic protein MID51 (smcr7l), partial CSB-YP391992DIL
CSB-EP391992DIL
CSB-BP391992DIL
CSB-MP391992DIL
CSB-EP391992DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Lysosome-associated membrane glycoprotein 5 (lamp5), partial CSB-YP391993XBF
CSB-EP391993XBF
CSB-BP391993XBF
CSB-MP391993XBF
CSB-EP391993XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0492 protein C20orf94 homolog CSB-YP391994XBF
CSB-EP391994XBF
CSB-BP391994XBF
CSB-MP391994XBF
CSB-EP391994XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0538 protein C2orf76 homolog CSB-YP391995XBF
CSB-EP391995XBF
CSB-BP391995XBF
CSB-MP391995XBF
CSB-EP391995XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Large proline-rich protein bag6 (Bag6), partial CSB-YP391996XBF
CSB-EP391996XBF
CSB-BP391996XBF
CSB-MP391996XBF
CSB-EP391996XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0500 protein C1orf216 homolog CSB-YP391997XBF
CSB-EP391997XBF
CSB-BP391997XBF
CSB-MP391997XBF
CSB-EP391997XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein DPCD (dpcd) CSB-YP391998XBF
CSB-EP391998XBF
CSB-BP391998XBF
CSB-MP391998XBF
CSB-EP391998XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ovarian cancer-associated gene 2 protein homolog (ovca2) CSB-YP391999XBF
CSB-EP391999XBF
CSB-BP391999XBF
CSB-MP391999XBF
CSB-EP391999XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Chromosomal replication initiator protein DnaA (dnaA) CSB-YP392000GEH
CSB-EP392000GEH
CSB-BP392000GEH
CSB-MP392000GEH
CSB-EP392000GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP392001GEH
CSB-EP392001GEH
CSB-BP392001GEH
CSB-MP392001GEH
CSB-EP392001GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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