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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium gilvum UPF0234 protein Mflv_5248 (Mflv_5248) CSB-YP392782MOK
CSB-EP392782MOK
CSB-BP392782MOK
CSB-MP392782MOK
CSB-EP392782MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP392783MOK
CSB-EP392783MOK
CSB-BP392783MOK
CSB-MP392783MOK
CSB-EP392783MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S7 (rpsG) CSB-YP392784MOK
CSB-EP392784MOK
CSB-BP392784MOK
CSB-MP392784MOK
CSB-EP392784MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L2 (rplB) CSB-YP392785MOK
CSB-EP392785MOK
CSB-BP392785MOK
CSB-MP392785MOK
CSB-EP392785MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S10 (rpsJ) CSB-YP392786MOK
CSB-EP392786MOK
CSB-BP392786MOK
CSB-MP392786MOK
CSB-EP392786MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP392787MOK
CSB-EP392787MOK
CSB-BP392787MOK
CSB-MP392787MOK
CSB-EP392787MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L27 (rpmA) CSB-YP392788MOK
CSB-EP392788MOK
CSB-BP392788MOK
CSB-MP392788MOK
CSB-EP392788MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Thiamine-phosphate synthase (thiE) CSB-YP392789MOK
CSB-EP392789MOK
CSB-BP392789MOK
CSB-MP392789MOK
CSB-EP392789MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 60 kDa chaperonin 1 (groL1), partial CSB-YP392790MOK
CSB-EP392790MOK
CSB-BP392790MOK
CSB-MP392790MOK
CSB-EP392790MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP392791MOK
CSB-EP392791MOK
CSB-BP392791MOK
CSB-MP392791MOK
CSB-EP392791MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum tRNA (guanine-N (7)-)-methyltransferase CSB-YP392792MOK
CSB-EP392792MOK
CSB-BP392792MOK
CSB-MP392792MOK
CSB-EP392792MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP392793MOK
CSB-EP392793MOK
CSB-BP392793MOK
CSB-MP392793MOK
CSB-EP392793MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Leucine--tRNA ligase (leuS), partial CSB-YP392794MOK
CSB-EP392794MOK
CSB-BP392794MOK
CSB-MP392794MOK
CSB-EP392794MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0310 protein Mflv_0785 (Mflv_0785) CSB-YP392795MOK
CSB-EP392795MOK
CSB-BP392795MOK
CSB-MP392795MOK
CSB-EP392795MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0271 protein Mflv_1451 (Mflv_1451) CSB-YP392796MOK
CSB-EP392796MOK
CSB-BP392796MOK
CSB-MP392796MOK
CSB-EP392796MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Cysteine--tRNA ligase (cysS) CSB-YP392797MOK
CSB-EP392797MOK
CSB-BP392797MOK
CSB-MP392797MOK
CSB-EP392797MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glycerol kinase (glpK), partial CSB-YP392798MOK
CSB-EP392798MOK
CSB-BP392798MOK
CSB-MP392798MOK
CSB-EP392798MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0133 protein Mflv_1287 (Mflv_1287) CSB-YP392799MOK
CSB-EP392799MOK
CSB-BP392799MOK
CSB-MP392799MOK
CSB-EP392799MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Bifunctional protein GlmU (glmU) CSB-YP392800MOK
CSB-EP392800MOK
CSB-BP392800MOK
CSB-MP392800MOK
CSB-EP392800MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP392801MOK
CSB-EP392801MOK
CSB-BP392801MOK
CSB-MP392801MOK
CSB-EP392801MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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