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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP391922MOK1
CSB-EP391922MOK1
CSB-BP391922MOK1
CSB-MP391922MOK1
CSB-EP391922MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP391923MOK
CSB-EP391923MOK
CSB-BP391923MOK
CSB-MP391923MOK
CSB-EP391923MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP391924MOK
CSB-EP391924MOK
CSB-BP391924MOK
CSB-MP391924MOK
CSB-EP391924MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum DNA ligase (ligA), partial CSB-YP391925MOK
CSB-EP391925MOK
CSB-BP391925MOK
CSB-MP391925MOK
CSB-EP391925MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP391926MOK
CSB-EP391926MOK
CSB-BP391926MOK
CSB-MP391926MOK
CSB-EP391926MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L19 (rplS) CSB-YP391927MOK
CSB-EP391927MOK
CSB-BP391927MOK
CSB-MP391927MOK
CSB-EP391927MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L29 (rpmC) CSB-YP391928MOK
CSB-EP391928MOK
CSB-BP391928MOK
CSB-MP391928MOK
CSB-EP391928MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S19 (rpsS) CSB-YP391929MOK
CSB-EP391929MOK
CSB-BP391929MOK
CSB-MP391929MOK
CSB-EP391929MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Putative S-adenosyl-L-methionine-dependent methyltransferase Mflv_5025 (Mflv_5025) CSB-YP391930MOK
CSB-EP391930MOK
CSB-BP391930MOK
CSB-MP391930MOK
CSB-EP391930MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Putative S-adenosyl-L-methionine-dependent methyltransferase Mflv_5024 (Mflv_5024) CSB-YP391931MOK
CSB-EP391931MOK
CSB-BP391931MOK
CSB-MP391931MOK
CSB-EP391931MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoglucosamine mutase (glmM) CSB-YP391932MOK
CSB-EP391932MOK
CSB-BP391932MOK
CSB-MP391932MOK
CSB-EP391932MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Maf-like protein Mflv_4776 (Mflv_4776) CSB-YP391933MOK
CSB-EP391933MOK
CSB-BP391933MOK
CSB-MP391933MOK
CSB-EP391933MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Small heat shock protein ibpA (ibpA) CSB-YP391934YAL
CSB-EP391934YAL
CSB-BP391934YAL
CSB-MP391934YAL
CSB-EP391934YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP391935YAL
CSB-EP391935YAL
CSB-BP391935YAL
CSB-MP391935YAL
CSB-EP391935YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Rhomboid protease glpG (glpG), partial CSB-YP391936YAL1
CSB-EP391936YAL1
CSB-BP391936YAL1
CSB-MP391936YAL1
CSB-EP391936YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ferrous iron transport protein C (feoC) CSB-YP391937YAL
CSB-EP391937YAL
CSB-BP391937YAL
CSB-MP391937YAL
CSB-EP391937YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein tsgA homolog (tsgA), partial CSB-YP391938YAL
CSB-EP391938YAL
CSB-BP391938YAL
CSB-MP391938YAL
CSB-EP391938YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0270 protein YPDSF_0104 (YPDSF_0104) CSB-YP391939YAL
CSB-EP391939YAL
CSB-BP391939YAL
CSB-MP391939YAL
CSB-EP391939YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Sulfurtransferase TusD (tusD) CSB-YP391940YAL
CSB-EP391940YAL
CSB-BP391940YAL
CSB-MP391940YAL
CSB-EP391940YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S12 (rpsL) CSB-YP391941YAL
CSB-EP391941YAL
CSB-BP391941YAL
CSB-MP391941YAL
CSB-EP391941YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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