重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Aeromonas salmonicida Sulfurtransferase TusA homolog (tusA) | CSB-YP392722AUI CSB-EP392722AUI CSB-BP392722AUI CSB-MP392722AUI CSB-EP392722AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) | CSB-YP392723AUI CSB-EP392723AUI CSB-BP392723AUI CSB-MP392723AUI CSB-EP392723AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Aeromonas salmonicida Ribosomal RNA small subunit methyltransferase G (rsmG) | CSB-YP392724AUI CSB-EP392724AUI CSB-BP392724AUI CSB-MP392724AUI CSB-EP392724AUI-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. Ribosomal RNA small subunit methyltransferase G (rsmG) | CSB-YP392725PYU CSB-EP392725PYU CSB-BP392725PYU CSB-MP392725PYU CSB-EP392725PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 50S ribosomal protein L11 (rplK) | CSB-YP392726PYU CSB-EP392726PYU CSB-BP392726PYU CSB-MP392726PYU CSB-EP392726PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. DNA-directed RNA polymerase subunit beta' (rpoC), partial | CSB-YP392727PYU CSB-EP392727PYU CSB-BP392727PYU CSB-MP392727PYU CSB-EP392727PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 50S ribosomal protein L23 (rplW) | CSB-YP392728PYU CSB-EP392728PYU CSB-BP392728PYU CSB-MP392728PYU CSB-EP392728PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S17 (rpsQ) | CSB-YP392729PYU CSB-EP392729PYU CSB-BP392729PYU CSB-MP392729PYU CSB-EP392729PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S8 (rpsH) | CSB-YP392730PYU CSB-EP392730PYU CSB-BP392730PYU CSB-MP392730PYU CSB-EP392730PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S5 (rpsE) | CSB-YP392731PYU CSB-EP392731PYU CSB-BP392731PYU CSB-MP392731PYU CSB-EP392731PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 50S ribosomal protein L15 (rplO) | CSB-YP392732PYU CSB-EP392732PYU CSB-BP392732PYU CSB-MP392732PYU CSB-EP392732PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 30S ribosomal protein S4 (rpsD) | CSB-YP392733PYU CSB-EP392733PYU CSB-BP392733PYU CSB-MP392733PYU CSB-EP392733PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. Imidazoleglycerol-phosphate dehydratase (hisB) | CSB-YP392734PYU CSB-EP392734PYU CSB-BP392734PYU CSB-MP392734PYU CSB-EP392734PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. Glutamyl-tRNA reductase (hemA) | CSB-YP392735PYU CSB-EP392735PYU CSB-BP392735PYU CSB-MP392735PYU CSB-EP392735PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial | CSB-YP392736PYU CSB-EP392736PYU CSB-BP392736PYU CSB-MP392736PYU CSB-EP392736PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) | CSB-YP392737PYU CSB-EP392737PYU CSB-BP392737PYU CSB-MP392737PYU CSB-EP392737PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. GTPase obg (obg) | CSB-YP392738PYU CSB-EP392738PYU CSB-BP392738PYU CSB-MP392738PYU CSB-EP392738PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 3-dehydroquinate dehydratase (aroQ) | CSB-YP392739PYU CSB-EP392739PYU CSB-BP392739PYU CSB-MP392739PYU CSB-EP392739PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. Gamma-glutamyl phosphate reductase (proA) | CSB-YP392740PYU CSB-EP392740PYU CSB-BP392740PYU CSB-MP392740PYU CSB-EP392740PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Polynucleobacter sp. 7-cyano-7-deazaguanine synthase (queC) | CSB-YP392741PYU CSB-EP392741PYU CSB-BP392741PYU CSB-MP392741PYU CSB-EP392741PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022