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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aeromonas salmonicida UPF0213 protein ASA_0550 (ASA_0550) CSB-YP392642AUI
CSB-EP392642AUI
CSB-BP392642AUI
CSB-MP392642AUI
CSB-EP392642AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP392643AUI
CSB-EP392643AUI
CSB-BP392643AUI
CSB-MP392643AUI
CSB-EP392643AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP392644AUI1
CSB-EP392644AUI1
CSB-BP392644AUI1
CSB-MP392644AUI1
CSB-EP392644AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S20 (rpsT) CSB-YP392645AUI
CSB-EP392645AUI
CSB-BP392645AUI
CSB-MP392645AUI
CSB-EP392645AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S6 (rpsF) CSB-YP392646AUI
CSB-EP392646AUI
CSB-BP392646AUI
CSB-MP392646AUI
CSB-EP392646AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP392647AUI
CSB-EP392647AUI
CSB-BP392647AUI
CSB-MP392647AUI
CSB-EP392647AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Sugar fermentation stimulation protein homolog (sfsA) CSB-YP392648AUI
CSB-EP392648AUI
CSB-BP392648AUI
CSB-MP392648AUI
CSB-EP392648AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Xanthine phosphoribosyltransferase (gpt) CSB-YP392649AUI
CSB-EP392649AUI
CSB-BP392649AUI
CSB-MP392649AUI
CSB-EP392649AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal protein L11 methyltransferase (prmA) CSB-YP392650AUI
CSB-EP392650AUI
CSB-BP392650AUI
CSB-MP392650AUI
CSB-EP392650AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP392651AUI
CSB-EP392651AUI
CSB-BP392651AUI
CSB-MP392651AUI
CSB-EP392651AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Large-conductance mechanosensitive channel (mscL), partial CSB-YP392652AUI1
CSB-EP392652AUI1
CSB-BP392652AUI1
CSB-MP392652AUI1
CSB-EP392652AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Leucine--tRNA ligase (leuS), partial CSB-YP392653AUI
CSB-EP392653AUI
CSB-BP392653AUI
CSB-MP392653AUI
CSB-EP392653AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida GMP reductase (guaC) CSB-YP392654AUI
CSB-EP392654AUI
CSB-BP392654AUI
CSB-MP392654AUI
CSB-EP392654AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Glutamyl-tRNA reductase (hemA) CSB-YP392655AUI
CSB-EP392655AUI
CSB-BP392655AUI
CSB-MP392655AUI
CSB-EP392655AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida DNA ligase (ligA), partial CSB-YP392656AUI
CSB-EP392656AUI
CSB-BP392656AUI
CSB-MP392656AUI
CSB-EP392656AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP392657AUI
CSB-EP392657AUI
CSB-BP392657AUI
CSB-MP392657AUI
CSB-EP392657AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP392658AUI
CSB-EP392658AUI
CSB-BP392658AUI
CSB-MP392658AUI
CSB-EP392658AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0434 protein ASA_1553 (ASA_1553) CSB-YP392659AUI
CSB-EP392659AUI
CSB-BP392659AUI
CSB-MP392659AUI
CSB-EP392659AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP392660AUI
CSB-EP392660AUI
CSB-BP392660AUI
CSB-MP392660AUI
CSB-EP392660AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP392661AUI
CSB-EP392661AUI
CSB-BP392661AUI
CSB-MP392661AUI
CSB-EP392661AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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