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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prosthecochloris vibrioformis Ribosome-binding factor A (rbfA) CSB-YP392622PZJ
CSB-EP392622PZJ
CSB-BP392622PZJ
CSB-MP392622PZJ
CSB-EP392622PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Ribosome maturation factor RimP (rimP) CSB-YP392623PZJ
CSB-EP392623PZJ
CSB-BP392623PZJ
CSB-MP392623PZJ
CSB-EP392623PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP392624PZJ
CSB-EP392624PZJ
CSB-BP392624PZJ
CSB-MP392624PZJ
CSB-EP392624PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 50S ribosomal protein L11 (rplK) CSB-YP392625PZJ
CSB-EP392625PZJ
CSB-BP392625PZJ
CSB-MP392625PZJ
CSB-EP392625PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis ATP synthase gamma chain (atpG) CSB-YP392626PZJ
CSB-EP392626PZJ
CSB-BP392626PZJ
CSB-MP392626PZJ
CSB-EP392626PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Homoserine kinase (thrB) CSB-YP392627PZJ
CSB-EP392627PZJ
CSB-BP392627PZJ
CSB-MP392627PZJ
CSB-EP392627PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Probable potassium transport system protein kup (kup), partial CSB-YP392628PZJ
CSB-EP392628PZJ
CSB-BP392628PZJ
CSB-MP392628PZJ
CSB-EP392628PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Orotate phosphoribosyltransferase (pyrE) CSB-YP392629PZJ
CSB-EP392629PZJ
CSB-BP392629PZJ
CSB-MP392629PZJ
CSB-EP392629PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Probable transaldolase (tal) CSB-YP392630PZJ
CSB-EP392630PZJ
CSB-BP392630PZJ
CSB-MP392630PZJ
CSB-EP392630PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP392631PZJ
CSB-EP392631PZJ
CSB-BP392631PZJ
CSB-MP392631PZJ
CSB-EP392631PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Protein MraZ (mraZ) CSB-YP392632PZJ
CSB-EP392632PZJ
CSB-BP392632PZJ
CSB-MP392632PZJ
CSB-EP392632PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Dihydroxy-acid dehydratase (ilvD), partial CSB-YP392633AUI
CSB-EP392633AUI
CSB-BP392633AUI
CSB-MP392633AUI
CSB-EP392633AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Aspartate carbamoyltransferase (pyrB) CSB-YP392634AUI
CSB-EP392634AUI
CSB-BP392634AUI
CSB-MP392634AUI
CSB-EP392634AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L11 (rplK) CSB-YP392635AUI
CSB-EP392635AUI
CSB-BP392635AUI
CSB-MP392635AUI
CSB-EP392635AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L7/L12 (rplL) CSB-YP392636AUI
CSB-EP392636AUI
CSB-BP392636AUI
CSB-MP392636AUI
CSB-EP392636AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S12 (rpsL) CSB-YP392637AUI
CSB-EP392637AUI
CSB-BP392637AUI
CSB-MP392637AUI
CSB-EP392637AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Lipid II flippase FtsW (ftsW), partial CSB-YP392638AUI
CSB-EP392638AUI
CSB-BP392638AUI
CSB-MP392638AUI
CSB-EP392638AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP392639AUI
CSB-EP392639AUI
CSB-BP392639AUI
CSB-MP392639AUI
CSB-EP392639AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Methylglyoxal synthase (mgsA) CSB-YP392640AUI
CSB-EP392640AUI
CSB-BP392640AUI
CSB-MP392640AUI
CSB-EP392640AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Multifunctional CCA protein (cca) CSB-YP392641AUI
CSB-EP392641AUI
CSB-BP392641AUI
CSB-MP392641AUI
CSB-EP392641AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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