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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum Ribosome maturation factor RimP (rimP) CSB-YP392822MOK
CSB-EP392822MOK
CSB-BP392822MOK
CSB-MP392822MOK
CSB-EP392822MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Diaminopimelate epimerase (dapF) CSB-YP392823MOK
CSB-EP392823MOK
CSB-BP392823MOK
CSB-MP392823MOK
CSB-EP392823MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP392824MOK
CSB-EP392824MOK
CSB-BP392824MOK
CSB-MP392824MOK
CSB-EP392824MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Threonine--tRNA ligase (thrS), partial CSB-YP392825MOK
CSB-EP392825MOK
CSB-BP392825MOK
CSB-MP392825MOK
CSB-EP392825MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP392826MOK
CSB-EP392826MOK
CSB-BP392826MOK
CSB-MP392826MOK
CSB-EP392826MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Protein nrdI (nrdI) CSB-YP392827MOK
CSB-EP392827MOK
CSB-BP392827MOK
CSB-MP392827MOK
CSB-EP392827MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ketol-acid reductoisomerase (ilvC) CSB-YP392828MOK
CSB-EP392828MOK
CSB-BP392828MOK
CSB-MP392828MOK
CSB-EP392828MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3-isopropylmalate dehydrogenase (leuB) CSB-YP392829MOK
CSB-EP392829MOK
CSB-BP392829MOK
CSB-MP392829MOK
CSB-EP392829MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP392830MOK
CSB-EP392830MOK
CSB-BP392830MOK
CSB-MP392830MOK
CSB-EP392830MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphopantetheine adenylyltransferase (coaD) CSB-YP392831MOK
CSB-EP392831MOK
CSB-BP392831MOK
CSB-MP392831MOK
CSB-EP392831MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S16 (rpsP) CSB-YP392832MOK
CSB-EP392832MOK
CSB-BP392832MOK
CSB-MP392832MOK
CSB-EP392832MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribonuclease HII (rnhB) CSB-YP392833MOK
CSB-EP392833MOK
CSB-BP392833MOK
CSB-MP392833MOK
CSB-EP392833MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L16 (rplP) CSB-YP392834MOK
CSB-EP392834MOK
CSB-BP392834MOK
CSB-MP392834MOK
CSB-EP392834MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L6 (rplF) CSB-YP392835MOK
CSB-EP392835MOK
CSB-BP392835MOK
CSB-MP392835MOK
CSB-EP392835MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L14 (rplN) CSB-YP392836MOK
CSB-EP392836MOK
CSB-BP392836MOK
CSB-MP392836MOK
CSB-EP392836MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S13 (rpsM) CSB-YP392837MOK
CSB-EP392837MOK
CSB-BP392837MOK
CSB-MP392837MOK
CSB-EP392837MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 30S ribosomal protein S4 (rpsD) CSB-YP392838MOK
CSB-EP392838MOK
CSB-BP392838MOK
CSB-MP392838MOK
CSB-EP392838MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP392839MOK
CSB-EP392839MOK
CSB-BP392839MOK
CSB-MP392839MOK
CSB-EP392839MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Bifunctional protein FolD (folD) CSB-YP392840MOK
CSB-EP392840MOK
CSB-BP392840MOK
CSB-MP392840MOK
CSB-EP392840MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP392841MOK
CSB-EP392841MOK
CSB-BP392841MOK
CSB-MP392841MOK
CSB-EP392841MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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