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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis Ribosome maturation factor RimP (rimP) CSB-YP394544YAL
CSB-EP394544YAL
CSB-BP394544YAL
CSB-MP394544YAL
CSB-EP394544YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 10 kDa chaperonin (groS) CSB-YP394545YAL
CSB-EP394545YAL
CSB-BP394545YAL
CSB-MP394545YAL
CSB-EP394545YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis LexA repressor (lexA) CSB-YP394546YAL
CSB-EP394546YAL
CSB-BP394546YAL
CSB-MP394546YAL
CSB-EP394546YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH pyrophosphatase (nudC) CSB-YP394547YAL
CSB-EP394547YAL
CSB-BP394547YAL
CSB-MP394547YAL
CSB-EP394547YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP394548YAL
CSB-EP394548YAL
CSB-BP394548YAL
CSB-MP394548YAL
CSB-EP394548YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Xylose isomerase (xylA) CSB-YP394549YAL
CSB-EP394549YAL
CSB-BP394549YAL
CSB-MP394549YAL
CSB-EP394549YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ATP-dependent protease subunit HslV (hslV) CSB-YP394550YAL
CSB-EP394550YAL
CSB-BP394550YAL
CSB-MP394550YAL
CSB-EP394550YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Orotate phosphoribosyltransferase (pyrE) CSB-YP394551YAL
CSB-EP394551YAL
CSB-BP394551YAL
CSB-MP394551YAL
CSB-EP394551YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Probable GTP-binding protein EngB (engB) CSB-YP394552YAL
CSB-EP394552YAL
CSB-BP394552YAL
CSB-MP394552YAL
CSB-EP394552YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP394553YAL
CSB-EP394553YAL
CSB-BP394553YAL
CSB-MP394553YAL
CSB-EP394553YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Virulence-associated V antigen (lcrV) CSB-YP394554YAL
CSB-EP394554YAL
CSB-BP394554YAL
CSB-MP394554YAL
CSB-EP394554YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Protein PB1-F2 (PB1-F2) CSB-YP394555ILR
CSB-EP394555ILR
CSB-BP394555ILR
CSB-MP394555ILR
CSB-EP394555ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP394556ILQ
CSB-EP394556ILQ
CSB-BP394556ILQ
CSB-MP394556ILQ
CSB-EP394556ILQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 1 Matrix protein (M) CSB-YP394557EJH
CSB-EP394557EJH
CSB-BP394557EJH
CSB-MP394557EJH
CSB-EP394557EJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 2 Matrix protein (M) CSB-YP394558EJI
CSB-EP394558EJI
CSB-BP394558EJI
CSB-MP394558EJI
CSB-EP394558EJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mauritiana Vitelline membrane protein Vm32E (Vm32E) CSB-YP394559DLT
CSB-EP394559DLT
CSB-BP394559DLT
CSB-MP394559DLT
CSB-EP394559DLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0505 protein C16orf62 homolog (zgc:163107), partial CSB-YP394560DIL
CSB-EP394560DIL
CSB-BP394560DIL
CSB-MP394560DIL
CSB-EP394560DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal protein S6 modification protein (rimK) CSB-YP394561PZP
CSB-EP394561PZP
CSB-BP394561PZP
CSB-MP394561PZP
CSB-EP394561PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP394562PZP
CSB-EP394562PZP
CSB-BP394562PZP
CSB-MP394562PZP
CSB-EP394562PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri D-amino acid dehydrogenase small subunit (dadA) CSB-YP394563PZP
CSB-EP394563PZP
CSB-BP394563PZP
CSB-MP394563PZP
CSB-EP394563PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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