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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP395344EIZ
CSB-EP395344EIZ
CSB-BP395344EIZ
CSB-MP395344EIZ
CSB-EP395344EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0259 membrane protein Ent638_2284 (Ent638_2284), partial CSB-YP395345EIZ1
CSB-EP395345EIZ1
CSB-BP395345EIZ1
CSB-MP395345EIZ1
CSB-EP395345EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Fatty acid metabolism regulator protein (fadR) CSB-YP395346EIZ
CSB-EP395346EIZ
CSB-BP395346EIZ
CSB-MP395346EIZ
CSB-EP395346EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP395347EIZ
CSB-EP395347EIZ
CSB-BP395347EIZ
CSB-MP395347EIZ
CSB-EP395347EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Copper homeostasis protein CutC (cutC) CSB-YP395348EIZ
CSB-EP395348EIZ
CSB-BP395348EIZ
CSB-MP395348EIZ
CSB-EP395348EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein dsrB (dsrB) CSB-YP395349EIZ
CSB-EP395349EIZ
CSB-BP395349EIZ
CSB-MP395349EIZ
CSB-EP395349EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Multidrug resistance protein MdtC (mdtC), partial CSB-YP395350EIZ
CSB-EP395350EIZ
CSB-BP395350EIZ
CSB-MP395350EIZ
CSB-EP395350EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Elongation factor P-like protein (Ent638_2766) CSB-YP395351EIZ
CSB-EP395351EIZ
CSB-BP395351EIZ
CSB-MP395351EIZ
CSB-EP395351EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0352 protein Ent638_2783 (Ent638_2783) CSB-YP395352EIZ
CSB-EP395352EIZ
CSB-BP395352EIZ
CSB-MP395352EIZ
CSB-EP395352EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP395353EIZ
CSB-EP395353EIZ
CSB-BP395353EIZ
CSB-MP395353EIZ
CSB-EP395353EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0304 protein Ent638_2838 (Ent638_2838) CSB-YP395354EIZ
CSB-EP395354EIZ
CSB-BP395354EIZ
CSB-MP395354EIZ
CSB-EP395354EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP395355EIZ
CSB-EP395355EIZ
CSB-BP395355EIZ
CSB-MP395355EIZ
CSB-EP395355EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Cell division protein ZipA homolog (zipA), partial CSB-YP395356EIZ
CSB-EP395356EIZ
CSB-BP395356EIZ
CSB-MP395356EIZ
CSB-EP395356EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP395357EIZ
CSB-EP395357EIZ
CSB-BP395357EIZ
CSB-MP395357EIZ
CSB-EP395357EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP395358EIZ
CSB-EP395358EIZ
CSB-BP395358EIZ
CSB-MP395358EIZ
CSB-EP395358EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Iron-binding protein IscA (iscA) CSB-YP395359EIZ
CSB-EP395359EIZ
CSB-BP395359EIZ
CSB-MP395359EIZ
CSB-EP395359EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP395360EIZ
CSB-EP395360EIZ
CSB-BP395360EIZ
CSB-MP395360EIZ
CSB-EP395360EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Regulatory protein recX (recX) CSB-YP395361EIZ
CSB-EP395361EIZ
CSB-BP395361EIZ
CSB-MP395361EIZ
CSB-EP395361EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 5'/3'-nucleotidase SurE (surE) CSB-YP395362EIZ
CSB-EP395362EIZ
CSB-BP395362EIZ
CSB-MP395362EIZ
CSB-EP395362EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP395363EIZ
CSB-EP395363EIZ
CSB-BP395363EIZ
CSB-MP395363EIZ
CSB-EP395363EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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