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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salinispora tropica 60 kDa chaperonin 1 (groL1), partial CSB-YP395444STD
CSB-EP395444STD
CSB-BP395444STD
CSB-MP395444STD
CSB-EP395444STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP395445STD
CSB-EP395445STD
CSB-BP395445STD
CSB-MP395445STD
CSB-EP395445STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Proteasome subunit beta 1 (prcB1) CSB-YP395446STD
CSB-EP395446STD
CSB-BP395446STD
CSB-MP395446STD
CSB-EP395446STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ketol-acid reductoisomerase (ilvC) CSB-YP395447STD
CSB-EP395447STD
CSB-BP395447STD
CSB-MP395447STD
CSB-EP395447STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S16 (rpsP) CSB-YP395448STD
CSB-EP395448STD
CSB-BP395448STD
CSB-MP395448STD
CSB-EP395448STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S2 (rpsB) CSB-YP395449STD
CSB-EP395449STD
CSB-BP395449STD
CSB-MP395449STD
CSB-EP395449STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S15 (rpsO) CSB-YP395450STD
CSB-EP395450STD
CSB-BP395450STD
CSB-MP395450STD
CSB-EP395450STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Probable transcriptional regulatory protein Strop_1792 (Strop_1792) CSB-YP395451STD
CSB-EP395451STD
CSB-BP395451STD
CSB-MP395451STD
CSB-EP395451STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Aspartate--tRNA ligase (aspS), partial CSB-YP395452STD
CSB-EP395452STD
CSB-BP395452STD
CSB-MP395452STD
CSB-EP395452STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica N utilization substance protein B homolog (nusB) CSB-YP395453STD
CSB-EP395453STD
CSB-BP395453STD
CSB-MP395453STD
CSB-EP395453STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica S-adenosylmethionine synthase (metK) CSB-YP395454STD
CSB-EP395454STD
CSB-BP395454STD
CSB-MP395454STD
CSB-EP395454STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Proteasome subunit beta 2 (prcB2) CSB-YP395455STD
CSB-EP395455STD
CSB-BP395455STD
CSB-MP395455STD
CSB-EP395455STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S14 (rpsN) CSB-YP395456STD
CSB-EP395456STD
CSB-BP395456STD
CSB-MP395456STD
CSB-EP395456STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica UPF0246 protein Strop_2927 (Strop_2927) CSB-YP395457STD
CSB-EP395457STD
CSB-BP395457STD
CSB-MP395457STD
CSB-EP395457STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Leucine--tRNA ligase (leuS), partial CSB-YP395458STD
CSB-EP395458STD
CSB-BP395458STD
CSB-MP395458STD
CSB-EP395458STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica GTPase obg (obg) CSB-YP395459STD
CSB-EP395459STD
CSB-BP395459STD
CSB-MP395459STD
CSB-EP395459STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP395460STD
CSB-EP395460STD
CSB-BP395460STD
CSB-MP395460STD
CSB-EP395460STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S9 (rpsI) CSB-YP395461STD
CSB-EP395461STD
CSB-BP395461STD
CSB-MP395461STD
CSB-EP395461STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S13 (rpsM) CSB-YP395462STD
CSB-EP395462STD
CSB-BP395462STD
CSB-MP395462STD
CSB-EP395462STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L6 (rplF) CSB-YP395463STD
CSB-EP395463STD
CSB-BP395463STD
CSB-MP395463STD
CSB-EP395463STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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