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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP396204YAL
CSB-EP396204YAL
CSB-BP396204YAL
CSB-MP396204YAL
CSB-EP396204YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 4-alpha-L-fucosyltransferase (wecF) CSB-YP396205YAL
CSB-EP396205YAL
CSB-BP396205YAL
CSB-MP396205YAL
CSB-EP396205YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP396206YAL
CSB-EP396206YAL
CSB-BP396206YAL
CSB-MP396206YAL
CSB-EP396206YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S15 (rpsO) CSB-YP396207YAL
CSB-EP396207YAL
CSB-BP396207YAL
CSB-MP396207YAL
CSB-EP396207YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP396208YAL
CSB-EP396208YAL
CSB-BP396208YAL
CSB-MP396208YAL
CSB-EP396208YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Divalent-cation tolerance protein CutA (cutA) CSB-YP396209YAL
CSB-EP396209YAL
CSB-BP396209YAL
CSB-MP396209YAL
CSB-EP396209YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Chorismate--pyruvate lyase (ubiC) CSB-YP396210YAL
CSB-EP396210YAL
CSB-BP396210YAL
CSB-MP396210YAL
CSB-EP396210YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 50S ribosomal protein L10 (rplJ) CSB-YP396211YAL
CSB-EP396211YAL
CSB-BP396211YAL
CSB-MP396211YAL
CSB-EP396211YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP396212YAL
CSB-EP396212YAL
CSB-BP396212YAL
CSB-MP396212YAL
CSB-EP396212YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0758 protein YPDSF_3856 (YPDSF_3856) CSB-YP396213YAL
CSB-EP396213YAL
CSB-BP396213YAL
CSB-MP396213YAL
CSB-EP396213YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0761 membrane protein YPDSF_3877 (YPDSF_3877), partial CSB-YP396214YAL1
CSB-EP396214YAL1
CSB-BP396214YAL1
CSB-MP396214YAL1
CSB-EP396214YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP396215YAL
CSB-EP396215YAL
CSB-BP396215YAL
CSB-MP396215YAL
CSB-EP396215YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Bifunctional protein GlmU (glmU) CSB-YP396216YAL
CSB-EP396216YAL
CSB-BP396216YAL
CSB-MP396216YAL
CSB-EP396216YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP396217ILR
CSB-EP396217ILR
CSB-BP396217ILR
CSB-MP396217ILR
CSB-EP396217ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP396218ILR
CSB-EP396218ILR
CSB-BP396218ILR
CSB-MP396218ILR
CSB-EP396218ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP396219ILQ
CSB-EP396219ILQ
CSB-BP396219ILQ
CSB-MP396219ILQ
CSB-EP396219ILQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP396220ILQ
CSB-EP396220ILQ
CSB-BP396220ILQ
CSB-MP396220ILQ
CSB-EP396220ILQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 2 Phosphoprotein (P) CSB-YP396221EJI
CSB-EP396221EJI
CSB-BP396221EJI
CSB-MP396221EJI
CSB-EP396221EJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila santomea Vitelline membrane protein Vm32E (Vm32E) CSB-YP396222DIF
CSB-EP396222DIF
CSB-BP396222DIF
CSB-MP396222DIF
CSB-EP396222DIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri L-seryl-tRNA (Sec) selenium transferase CSB-YP396223PZP
CSB-EP396223PZP
CSB-BP396223PZP
CSB-MP396223PZP
CSB-EP396223PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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