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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L18P (rpl18p) CSB-YP396404PZU
CSB-EP396404PZU
CSB-BP396404PZU
CSB-MP396404PZU
CSB-EP396404PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L10e (rpl10e) CSB-YP396405PZU
CSB-EP396405PZU
CSB-BP396405PZU
CSB-MP396405PZU
CSB-EP396405PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Tyrosine--tRNA ligase (tyrS) CSB-YP396406PZU
CSB-EP396406PZU
CSB-BP396406PZU
CSB-MP396406PZU
CSB-EP396406PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Elongation factor 1-beta (ef1b) CSB-YP396407PZU
CSB-EP396407PZU
CSB-BP396407PZU
CSB-MP396407PZU
CSB-EP396407PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP396408RLG
CSB-EP396408RLG
CSB-BP396408RLG
CSB-MP396408RLG
CSB-EP396408RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribonuclease HII (rnhB) CSB-YP396409RLG
CSB-EP396409RLG
CSB-BP396409RLG
CSB-MP396409RLG
CSB-EP396409RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides SsrA-binding protein (smpB) CSB-YP396410RLG
CSB-EP396410RLG
CSB-BP396410RLG
CSB-MP396410RLG
CSB-EP396410RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Dihydroorotase (pyrC) CSB-YP396411RLG
CSB-EP396411RLG
CSB-BP396411RLG
CSB-MP396411RLG
CSB-EP396411RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Cobyric acid synthase (cobQ) CSB-YP396412RLG
CSB-EP396412RLG
CSB-BP396412RLG
CSB-MP396412RLG
CSB-EP396412RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP396413RLG
CSB-EP396413RLG
CSB-BP396413RLG
CSB-MP396413RLG
CSB-EP396413RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP396414RLG
CSB-EP396414RLG
CSB-BP396414RLG
CSB-MP396414RLG
CSB-EP396414RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase (ilvC) CSB-YP396415RLG
CSB-EP396415RLG
CSB-BP396415RLG
CSB-MP396415RLG
CSB-EP396415RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP396416RLG
CSB-EP396416RLG
CSB-BP396416RLG
CSB-MP396416RLG
CSB-EP396416RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Adenylosuccinate synthetase (purA) CSB-YP396417RLG
CSB-EP396417RLG
CSB-BP396417RLG
CSB-MP396417RLG
CSB-EP396417RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Arginine--tRNA ligase (argS), partial CSB-YP396418RLG
CSB-EP396418RLG
CSB-BP396418RLG
CSB-MP396418RLG
CSB-EP396418RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Tyrosine--tRNA ligase (tyrS) CSB-YP396419RLG
CSB-EP396419RLG
CSB-BP396419RLG
CSB-MP396419RLG
CSB-EP396419RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Light-independent protochlorophyllide reductase subunit N (bchN) CSB-YP396420RLG
CSB-EP396420RLG
CSB-BP396420RLG
CSB-MP396420RLG
CSB-EP396420RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glycogen synthase (glgA) CSB-YP396421RLG
CSB-EP396421RLG
CSB-BP396421RLG
CSB-MP396421RLG
CSB-EP396421RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP396422RLG
CSB-EP396422RLG
CSB-BP396422RLG
CSB-MP396422RLG
CSB-EP396422RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP396423RLG
CSB-EP396423RLG
CSB-BP396423RLG
CSB-MP396423RLG
CSB-EP396423RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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