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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus suis Pantothenate kinase (coaA) CSB-YP394624SUS
CSB-EP394624SUS
CSB-BP394624SUS
CSB-MP394624SUS
CSB-EP394624SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Shikimate dehydrogenase (aroE) CSB-YP394625SUS
CSB-EP394625SUS
CSB-BP394625SUS
CSB-MP394625SUS
CSB-EP394625SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0346 protein SSU05_1322 (SSU05_1322) CSB-YP394626SUS
CSB-EP394626SUS
CSB-BP394626SUS
CSB-MP394626SUS
CSB-EP394626SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis SsrA-binding protein (smpB) CSB-YP394627SUS
CSB-EP394627SUS
CSB-BP394627SUS
CSB-MP394627SUS
CSB-EP394627SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribose-5-phosphate isomerase A (rpiA) CSB-YP394628SUS
CSB-EP394628SUS
CSB-BP394628SUS
CSB-MP394628SUS
CSB-EP394628SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Transcription elongation factor GreA (greA) CSB-YP394629SUS
CSB-EP394629SUS
CSB-BP394629SUS
CSB-MP394629SUS
CSB-EP394629SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Glutamate--tRNA ligase (gltX) CSB-YP394630SUS
CSB-EP394630SUS
CSB-BP394630SUS
CSB-MP394630SUS
CSB-EP394630SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP394631SUS
CSB-EP394631SUS
CSB-BP394631SUS
CSB-MP394631SUS
CSB-EP394631SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP394632SUT
CSB-EP394632SUT
CSB-BP394632SUT
CSB-MP394632SUT
CSB-EP394632SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S19 (rpsS) CSB-YP394633SUT
CSB-EP394633SUT
CSB-BP394633SUT
CSB-MP394633SUT
CSB-EP394633SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S11 (rpsK) CSB-YP394634SUT
CSB-EP394634SUT
CSB-BP394634SUT
CSB-MP394634SUT
CSB-EP394634SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis DNA polymerase IV (dinB) CSB-YP394635SUT
CSB-EP394635SUT
CSB-BP394635SUT
CSB-MP394635SUT
CSB-EP394635SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP394636SUT
CSB-EP394636SUT
CSB-BP394636SUT
CSB-MP394636SUT
CSB-EP394636SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP394637SUT
CSB-EP394637SUT
CSB-BP394637SUT
CSB-MP394637SUT
CSB-EP394637SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP394638SUT
CSB-EP394638SUT
CSB-BP394638SUT
CSB-MP394638SUT
CSB-EP394638SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Shikimate kinase (aroK) CSB-YP394639SUT
CSB-EP394639SUT
CSB-BP394639SUT
CSB-MP394639SUT
CSB-EP394639SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Dihydrodipicolinate synthase (dapA) CSB-YP394640SUT
CSB-EP394640SUT
CSB-BP394640SUT
CSB-MP394640SUT
CSB-EP394640SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Pyrrolidone-carboxylate peptidase (pcp) CSB-YP394641SUT
CSB-EP394641SUT
CSB-BP394641SUT
CSB-MP394641SUT
CSB-EP394641SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribonuclease HII (rnhB) CSB-YP394642SUT
CSB-EP394642SUT
CSB-BP394642SUT
CSB-MP394642SUT
CSB-EP394642SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Deoxyribose-phosphate aldolase (deoC) CSB-YP394643SUT
CSB-EP394643SUT
CSB-BP394643SUT
CSB-MP394643SUT
CSB-EP394643SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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