Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus suis DNA polymerase III polC-type (polC), partial CSB-YP395984SUT
CSB-EP395984SUT
CSB-BP395984SUT
CSB-MP395984SUT
CSB-EP395984SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S2 (rpsB) CSB-YP395985SUT
CSB-EP395985SUT
CSB-BP395985SUT
CSB-MP395985SUT
CSB-EP395985SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP395986SUT
CSB-EP395986SUT
CSB-BP395986SUT
CSB-MP395986SUT
CSB-EP395986SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP395987SUT
CSB-EP395987SUT
CSB-BP395987SUT
CSB-MP395987SUT
CSB-EP395987SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP395988SUT
CSB-EP395988SUT
CSB-BP395988SUT
CSB-MP395988SUT
CSB-EP395988SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. DNA ligase B (ligB), partial CSB-YP395989EIZ
CSB-EP395989EIZ
CSB-BP395989EIZ
CSB-MP395989EIZ
CSB-EP395989EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP395990EIZ
CSB-EP395990EIZ
CSB-BP395990EIZ
CSB-MP395990EIZ
CSB-EP395990EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L1 (rplA) CSB-YP395991EIZ
CSB-EP395991EIZ
CSB-BP395991EIZ
CSB-MP395991EIZ
CSB-EP395991EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. NADH pyrophosphatase (nudC) CSB-YP395992EIZ
CSB-EP395992EIZ
CSB-BP395992EIZ
CSB-MP395992EIZ
CSB-EP395992EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP395993EIZ
CSB-EP395993EIZ
CSB-BP395993EIZ
CSB-MP395993EIZ
CSB-EP395993EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L9 (rplI) CSB-YP395994EIZ
CSB-EP395994EIZ
CSB-BP395994EIZ
CSB-MP395994EIZ
CSB-EP395994EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP395995EIZ
CSB-EP395995EIZ
CSB-BP395995EIZ
CSB-MP395995EIZ
CSB-EP395995EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Peptide chain release factor 3 (prfC), partial CSB-YP395996EIZ
CSB-EP395996EIZ
CSB-BP395996EIZ
CSB-MP395996EIZ
CSB-EP395996EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Chaperone protein DnaJ (dnaJ) CSB-YP395997EIZ
CSB-EP395997EIZ
CSB-BP395997EIZ
CSB-MP395997EIZ
CSB-EP395997EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP395998EIZ
CSB-EP395998EIZ
CSB-BP395998EIZ
CSB-MP395998EIZ
CSB-EP395998EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP395999EIZ
CSB-EP395999EIZ
CSB-BP395999EIZ
CSB-MP395999EIZ
CSB-EP395999EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. DNA gyrase inhibitor YacG (yacG) CSB-YP396000EIZ
CSB-EP396000EIZ
CSB-BP396000EIZ
CSB-MP396000EIZ
CSB-EP396000EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP396001EIZ
CSB-EP396001EIZ
CSB-BP396001EIZ
CSB-MP396001EIZ
CSB-EP396001EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP396002EIZ
CSB-EP396002EIZ
CSB-BP396002EIZ
CSB-MP396002EIZ
CSB-EP396002EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Proline--tRNA ligase (proS), partial CSB-YP396003EIZ
CSB-EP396003EIZ
CSB-BP396003EIZ
CSB-MP396003EIZ
CSB-EP396003EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>