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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens Shikimate kinase (aroK) CSB-YP396584STQ
CSB-EP396584STQ
CSB-BP396584STQ
CSB-MP396584STQ
CSB-EP396584STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP396585STQ
CSB-EP396585STQ
CSB-BP396585STQ
CSB-MP396585STQ
CSB-EP396585STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L30 (rpmD) CSB-YP396586STQ
CSB-EP396586STQ
CSB-BP396586STQ
CSB-MP396586STQ
CSB-EP396586STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L24 (rplX) CSB-YP396587STQ
CSB-EP396587STQ
CSB-BP396587STQ
CSB-MP396587STQ
CSB-EP396587STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L16 (rplP) CSB-YP396588STQ
CSB-EP396588STQ
CSB-BP396588STQ
CSB-MP396588STQ
CSB-EP396588STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP396589STQ
CSB-EP396589STQ
CSB-BP396589STQ
CSB-MP396589STQ
CSB-EP396589STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens LexA repressor (lexA) CSB-YP396590STQ
CSB-EP396590STQ
CSB-BP396590STQ
CSB-MP396590STQ
CSB-EP396590STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Bifunctional protein GlmU (glmU) CSB-YP396591STQ
CSB-EP396591STQ
CSB-BP396591STQ
CSB-MP396591STQ
CSB-EP396591STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA modification GTPase MnmE (mnmE) CSB-YP396592STQ
CSB-EP396592STQ
CSB-BP396592STQ
CSB-MP396592STQ
CSB-EP396592STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Putative membrane protein insertion efficiency factor (Sputcn32_4003) CSB-YP396593STQ
CSB-EP396593STQ
CSB-BP396593STQ
CSB-MP396593STQ
CSB-EP396593STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP396594MNM
CSB-EP396594MNM
CSB-BP396594MNM
CSB-MP396594MNM
CSB-EP396594MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula DNA-directed RNA polymerase subunit H (rpoH) CSB-YP396595MNM
CSB-EP396595MNM
CSB-BP396595MNM
CSB-MP396595MNM
CSB-EP396595MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S19P (rps19p) CSB-YP396596MNM
CSB-EP396596MNM
CSB-BP396596MNM
CSB-MP396596MNM
CSB-EP396596MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 50S ribosomal protein L22P (rpl22p) CSB-YP396597MNM
CSB-EP396597MNM
CSB-BP396597MNM
CSB-MP396597MNM
CSB-EP396597MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S8e (rps8e) CSB-YP396598MNM
CSB-EP396598MNM
CSB-BP396598MNM
CSB-MP396598MNM
CSB-EP396598MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula DNA polymerase IV (dbh) CSB-YP396599MNM
CSB-EP396599MNM
CSB-BP396599MNM
CSB-MP396599MNM
CSB-EP396599MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Nucleoside-triphosphatase THEP1 (Msed_0721) CSB-YP396600MNM
CSB-EP396600MNM
CSB-BP396600MNM
CSB-MP396600MNM
CSB-EP396600MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Phosphoenolpyruvate carboxylase (ppcA), partial CSB-YP396601MNM
CSB-EP396601MNM
CSB-BP396601MNM
CSB-MP396601MNM
CSB-EP396601MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 3-hydroxypropionyl-coenzyme A synthetase (Msed_1456), partial CSB-YP396602MNM
CSB-EP396602MNM
CSB-BP396602MNM
CSB-MP396602MNM
CSB-EP396602MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Preprotein translocase subunit secE (secE), partial CSB-YP396603MNM
CSB-EP396603MNM
CSB-BP396603MNM
CSB-MP396603MNM
CSB-EP396603MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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