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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum arsenaticum V-type ATP synthase alpha chain (atpA), partial CSB-YP395744PZU
CSB-EP395744PZU
CSB-BP395744PZU
CSB-MP395744PZU
CSB-EP395744PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0246 protein Rsph17025_0016 (Rsph17025_0016) CSB-YP395745RLG
CSB-EP395745RLG
CSB-BP395745RLG
CSB-MP395745RLG
CSB-EP395745RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP395746RLG
CSB-EP395746RLG
CSB-BP395746RLG
CSB-MP395746RLG
CSB-EP395746RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L19 (rplS) CSB-YP395747RLG
CSB-EP395747RLG
CSB-BP395747RLG
CSB-MP395747RLG
CSB-EP395747RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Aspartate carbamoyltransferase (pyrB) CSB-YP395748RLG
CSB-EP395748RLG
CSB-BP395748RLG
CSB-MP395748RLG
CSB-EP395748RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glutamate--tRNA ligase 1 (gltX1) CSB-YP395749RLG
CSB-EP395749RLG
CSB-BP395749RLG
CSB-MP395749RLG
CSB-EP395749RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Bifunctional protein FolD (folD) CSB-YP395750RLG
CSB-EP395750RLG
CSB-BP395750RLG
CSB-MP395750RLG
CSB-EP395750RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable transcriptional regulatory protein Rsph17025_0577 (Rsph17025_0577) CSB-YP395751RLG
CSB-EP395751RLG
CSB-BP395751RLG
CSB-MP395751RLG
CSB-EP395751RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP395752RLG
CSB-EP395752RLG
CSB-BP395752RLG
CSB-MP395752RLG
CSB-EP395752RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Transcriptional repressor NrdR (nrdR) CSB-YP395753RLG
CSB-EP395753RLG
CSB-BP395753RLG
CSB-MP395753RLG
CSB-EP395753RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP395754RLG
CSB-EP395754RLG
CSB-BP395754RLG
CSB-MP395754RLG
CSB-EP395754RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Urease subunit beta (ureB) CSB-YP395755RLG
CSB-EP395755RLG
CSB-BP395755RLG
CSB-MP395755RLG
CSB-EP395755RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP395756RLG
CSB-EP395756RLG
CSB-BP395756RLG
CSB-MP395756RLG
CSB-EP395756RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP395757RLG
CSB-EP395757RLG
CSB-BP395757RLG
CSB-MP395757RLG
CSB-EP395757RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides DNA ligase (ligA), partial CSB-YP395758RLG
CSB-EP395758RLG
CSB-BP395758RLG
CSB-MP395758RLG
CSB-EP395758RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S2 (rpsB) CSB-YP395759RLG
CSB-EP395759RLG
CSB-BP395759RLG
CSB-MP395759RLG
CSB-EP395759RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP395760RLG
CSB-EP395760RLG
CSB-BP395760RLG
CSB-MP395760RLG
CSB-EP395760RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP395761RLG1
CSB-EP395761RLG1
CSB-BP395761RLG1
CSB-MP395761RLG1
CSB-EP395761RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP395762RLG
CSB-EP395762RLG
CSB-BP395762RLG
CSB-MP395762RLG
CSB-EP395762RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S9 (rpsI) CSB-YP395763RLG
CSB-EP395763RLG
CSB-BP395763RLG
CSB-MP395763RLG
CSB-EP395763RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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