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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides Type III pantothenate kinase (coaX) CSB-YP395424RLG
CSB-EP395424RLG
CSB-BP395424RLG
CSB-MP395424RLG
CSB-EP395424RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP395425RLG
CSB-EP395425RLG
CSB-BP395425RLG
CSB-MP395425RLG
CSB-EP395425RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Uridylate kinase (pyrH) CSB-YP395426RLG
CSB-EP395426RLG
CSB-BP395426RLG
CSB-MP395426RLG
CSB-EP395426RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S4 (rpsD) CSB-YP395427RLG
CSB-EP395427RLG
CSB-BP395427RLG
CSB-MP395427RLG
CSB-EP395427RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP395428RLG
CSB-EP395428RLG
CSB-BP395428RLG
CSB-MP395428RLG
CSB-EP395428RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0145 protein Rsph17025_2361 (Rsph17025_2361) CSB-YP395429RLG
CSB-EP395429RLG
CSB-BP395429RLG
CSB-MP395429RLG
CSB-EP395429RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Large-conductance mechanosensitive channel (mscL), partial CSB-YP395430RLG1
CSB-EP395430RLG1
CSB-BP395430RLG1
CSB-MP395430RLG1
CSB-EP395430RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S11 (rpsK) CSB-YP395431RLG
CSB-EP395431RLG
CSB-BP395431RLG
CSB-MP395431RLG
CSB-EP395431RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L6 (rplF) CSB-YP395432RLG
CSB-EP395432RLG
CSB-BP395432RLG
CSB-MP395432RLG
CSB-EP395432RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP395433RLG
CSB-EP395433RLG
CSB-BP395433RLG
CSB-MP395433RLG
CSB-EP395433RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0042 nucleotide-binding protein Rsph17025_2562 (Rsph17025_2562) CSB-YP395434RLG
CSB-EP395434RLG
CSB-BP395434RLG
CSB-MP395434RLG
CSB-EP395434RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP395435RLG
CSB-EP395435RLG
CSB-BP395435RLG
CSB-MP395435RLG
CSB-EP395435RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP395436RLG
CSB-EP395436RLG
CSB-BP395436RLG
CSB-MP395436RLG
CSB-EP395436RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosome maturation factor RimP (rimP) CSB-YP395437RLG
CSB-EP395437RLG
CSB-BP395437RLG
CSB-MP395437RLG
CSB-EP395437RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L36 (rpmJ) CSB-YP395438RLG
CSB-EP395438RLG
CSB-BP395438RLG
CSB-MP395438RLG
CSB-EP395438RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0434 protein Rsph17025_2896 (Rsph17025_2896) CSB-YP395439RLG
CSB-EP395439RLG
CSB-BP395439RLG
CSB-MP395439RLG
CSB-EP395439RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA pseudouridine synthase B (truB) CSB-YP395440RLG
CSB-EP395440RLG
CSB-BP395440RLG
CSB-MP395440RLG
CSB-EP395440RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Elongation factor 4 (lepA), partial CSB-YP395441RLG
CSB-EP395441RLG
CSB-BP395441RLG
CSB-MP395441RLG
CSB-EP395441RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Catalase-peroxidase (katG), partial CSB-YP395442RLG
CSB-EP395442RLG
CSB-BP395442RLG
CSB-MP395442RLG
CSB-EP395442RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP395443RLG
CSB-EP395443RLG
CSB-BP395443RLG
CSB-MP395443RLG
CSB-EP395443RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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