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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus suis Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP395944SUS
CSB-EP395944SUS
CSB-BP395944SUS
CSB-MP395944SUS
CSB-EP395944SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP395945SUS
CSB-EP395945SUS
CSB-BP395945SUS
CSB-MP395945SUS
CSB-EP395945SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP395946SUS
CSB-EP395946SUS
CSB-BP395946SUS
CSB-MP395946SUS
CSB-EP395946SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Dihydrodipicolinate reductase (dapB) CSB-YP395947SUS
CSB-EP395947SUS
CSB-BP395947SUS
CSB-MP395947SUS
CSB-EP395947SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Tyrosine recombinase XerS (xerS) CSB-YP395948SUS
CSB-EP395948SUS
CSB-BP395948SUS
CSB-MP395948SUS
CSB-EP395948SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis L-lactate dehydrogenase (ldh) CSB-YP395949SUS
CSB-EP395949SUS
CSB-BP395949SUS
CSB-MP395949SUS
CSB-EP395949SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis ATP synthase gamma chain (atpG) CSB-YP395950SUS
CSB-EP395950SUS
CSB-BP395950SUS
CSB-MP395950SUS
CSB-EP395950SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 3-dehydroquinate dehydratase (aroD) CSB-YP395951SUS
CSB-EP395951SUS
CSB-BP395951SUS
CSB-MP395951SUS
CSB-EP395951SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L11 (rplK) CSB-YP395952SUS
CSB-EP395952SUS
CSB-BP395952SUS
CSB-MP395952SUS
CSB-EP395952SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis tRNA modification GTPase MnmE (mnmE) CSB-YP395953SUS
CSB-EP395953SUS
CSB-BP395953SUS
CSB-MP395953SUS
CSB-EP395953SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Protein sprT-like (SSU05_1611) CSB-YP395954SUS
CSB-EP395954SUS
CSB-BP395954SUS
CSB-MP395954SUS
CSB-EP395954SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP395955SUS
CSB-EP395955SUS
CSB-BP395955SUS
CSB-MP395955SUS
CSB-EP395955SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S18 (rpsR) CSB-YP395956SUS
CSB-EP395956SUS
CSB-BP395956SUS
CSB-MP395956SUS
CSB-EP395956SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP395957SUS
CSB-EP395957SUS
CSB-BP395957SUS
CSB-MP395957SUS
CSB-EP395957SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ferredoxin--NADP reductase (SSU05_1986) CSB-YP395958SUS
CSB-EP395958SUS
CSB-BP395958SUS
CSB-MP395958SUS
CSB-EP395958SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Leucine--tRNA ligase (leuS), partial CSB-YP395959SUS
CSB-EP395959SUS
CSB-BP395959SUS
CSB-MP395959SUS
CSB-EP395959SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP395960SUS
CSB-EP395960SUS
CSB-BP395960SUS
CSB-MP395960SUS
CSB-EP395960SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP395961SUT
CSB-EP395961SUT
CSB-BP395961SUT
CSB-MP395961SUT
CSB-EP395961SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L4 (rplD) CSB-YP395962SUT
CSB-EP395962SUT
CSB-BP395962SUT
CSB-MP395962SUT
CSB-EP395962SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S17 (rpsQ) CSB-YP395963SUT
CSB-EP395963SUT
CSB-BP395963SUT
CSB-MP395963SUT
CSB-EP395963SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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