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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caldicellulosiruptor saccharolyticus Cysteine--tRNA ligase (cysS) CSB-YP396144CVX
CSB-EP396144CVX
CSB-BP396144CVX
CSB-MP396144CVX
CSB-EP396144CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus S-adenosylmethionine synthase (metK) CSB-YP396145CVX
CSB-EP396145CVX
CSB-BP396145CVX
CSB-MP396145CVX
CSB-EP396145CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus GTPase Der (der) CSB-YP396146CVX
CSB-EP396146CVX
CSB-BP396146CVX
CSB-MP396146CVX
CSB-EP396146CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP396147CVX
CSB-EP396147CVX
CSB-BP396147CVX
CSB-MP396147CVX
CSB-EP396147CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0735 ACT domain-containing protein Csac_0995 (Csac_0995) CSB-YP396148CVX
CSB-EP396148CVX
CSB-BP396148CVX
CSB-MP396148CVX
CSB-EP396148CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Shikimate dehydrogenase (aroE) CSB-YP396149CVX
CSB-EP396149CVX
CSB-BP396149CVX
CSB-MP396149CVX
CSB-EP396149CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UvrABC system protein C (uvrC), partial CSB-YP396150CVX
CSB-EP396150CVX
CSB-BP396150CVX
CSB-MP396150CVX
CSB-EP396150CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP396151CVX
CSB-EP396151CVX
CSB-BP396151CVX
CSB-MP396151CVX
CSB-EP396151CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0210 protein Csac_1314 (Csac_1314) CSB-YP396152CVX
CSB-EP396152CVX
CSB-BP396152CVX
CSB-MP396152CVX
CSB-EP396152CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L13 (rplM) CSB-YP396153CVX
CSB-EP396153CVX
CSB-BP396153CVX
CSB-MP396153CVX
CSB-EP396153CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP396154CVX
CSB-EP396154CVX
CSB-BP396154CVX
CSB-MP396154CVX
CSB-EP396154CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus GTPase obg (obg) CSB-YP396155CVX
CSB-EP396155CVX
CSB-BP396155CVX
CSB-MP396155CVX
CSB-EP396155CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP396156CVX
CSB-EP396156CVX
CSB-BP396156CVX
CSB-MP396156CVX
CSB-EP396156CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Chaperone protein DnaK (dnaK), partial CSB-YP396157CVX
CSB-EP396157CVX
CSB-BP396157CVX
CSB-MP396157CVX
CSB-EP396157CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L35 (rpmI) CSB-YP396158CVX
CSB-EP396158CVX
CSB-BP396158CVX
CSB-MP396158CVX
CSB-EP396158CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Quinolinate synthase A (nadA) CSB-YP396159CVX
CSB-EP396159CVX
CSB-BP396159CVX
CSB-MP396159CVX
CSB-EP396159CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Leucine--tRNA ligase (leuS), partial CSB-YP396160CVX
CSB-EP396160CVX
CSB-BP396160CVX
CSB-MP396160CVX
CSB-EP396160CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Aspartate carbamoyltransferase (pyrB) CSB-YP396161CVX
CSB-EP396161CVX
CSB-BP396161CVX
CSB-MP396161CVX
CSB-EP396161CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP396162CVX
CSB-EP396162CVX
CSB-BP396162CVX
CSB-MP396162CVX
CSB-EP396162CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Transcriptional repressor NrdR (nrdR) CSB-YP396163CVX
CSB-EP396163CVX
CSB-BP396163CVX
CSB-MP396163CVX
CSB-EP396163CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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