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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas mendocina UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP396925PZM
CSB-EP396925PZM
CSB-BP396925PZM
CSB-MP396925PZM
CSB-EP396925PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 30S ribosomal protein S20 (rpsT) CSB-YP396926PZM
CSB-EP396926PZM
CSB-BP396926PZM
CSB-MP396926PZM
CSB-EP396926PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Peptidyl-tRNA hydrolase (pth) CSB-YP396927PZM
CSB-EP396927PZM
CSB-BP396927PZM
CSB-MP396927PZM
CSB-EP396927PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Glutamate racemase (murI) CSB-YP396928PZM
CSB-EP396928PZM
CSB-BP396928PZM
CSB-MP396928PZM
CSB-EP396928PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Proline--tRNA ligase (proS), partial CSB-YP396929PZM
CSB-EP396929PZM
CSB-BP396929PZM
CSB-MP396929PZM
CSB-EP396929PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Methionine--tRNA ligase (metG), partial CSB-YP396930PZM
CSB-EP396930PZM
CSB-BP396930PZM
CSB-MP396930PZM
CSB-EP396930PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UPF0434 protein Pmen_1615 (Pmen_1615) CSB-YP396931PZM
CSB-EP396931PZM
CSB-BP396931PZM
CSB-MP396931PZM
CSB-EP396931PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UPF0176 protein Pmen_1693 (Pmen_1693) CSB-YP396932PZM
CSB-EP396932PZM
CSB-BP396932PZM
CSB-MP396932PZM
CSB-EP396932PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP396933PZM
CSB-EP396933PZM
CSB-BP396933PZM
CSB-MP396933PZM
CSB-EP396933PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Chorismate synthase (aroC) CSB-YP396934PZM
CSB-EP396934PZM
CSB-BP396934PZM
CSB-MP396934PZM
CSB-EP396934PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L20 (rplT) CSB-YP396935PZM
CSB-EP396935PZM
CSB-BP396935PZM
CSB-MP396935PZM
CSB-EP396935PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP396936PZM
CSB-EP396936PZM
CSB-BP396936PZM
CSB-MP396936PZM
CSB-EP396936PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Putative regulator of ribonuclease activity (Pmen_2087) CSB-YP396937PZM
CSB-EP396937PZM
CSB-BP396937PZM
CSB-MP396937PZM
CSB-EP396937PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Undecaprenyl-diphosphatase (uppP), partial CSB-YP396938PZM1
CSB-EP396938PZM1
CSB-BP396938PZM1
CSB-MP396938PZM1
CSB-EP396938PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP396939PZM
CSB-EP396939PZM
CSB-BP396939PZM
CSB-MP396939PZM
CSB-EP396939PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cysteine--tRNA ligase (cysS) CSB-YP396940PZM
CSB-EP396940PZM
CSB-BP396940PZM
CSB-MP396940PZM
CSB-EP396940PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP396941PZM
CSB-EP396941PZM
CSB-BP396941PZM
CSB-MP396941PZM
CSB-EP396941PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP396942PZM
CSB-EP396942PZM
CSB-BP396942PZM
CSB-MP396942PZM
CSB-EP396942PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Dihydroorotate dehydrogenase (quinone) CSB-YP396943PZM
CSB-EP396943PZM
CSB-BP396943PZM
CSB-MP396943PZM
CSB-EP396943PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Regulatory protein recX (recX) CSB-YP396944PZM
CSB-EP396944PZM
CSB-BP396944PZM
CSB-MP396944PZM
CSB-EP396944PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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