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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas mendocina Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP397526PZM1
CSB-EP397526PZM1
CSB-BP397526PZM1
CSB-MP397526PZM1
CSB-EP397526PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP397527PZM
CSB-EP397527PZM
CSB-BP397527PZM
CSB-MP397527PZM
CSB-EP397527PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP397528PZM
CSB-EP397528PZM
CSB-BP397528PZM
CSB-MP397528PZM
CSB-EP397528PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Integration host factor subunit alpha (ihfA) CSB-YP397529PZM
CSB-EP397529PZM
CSB-BP397529PZM
CSB-MP397529PZM
CSB-EP397529PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Siroheme synthase (cysG) CSB-YP397530PZM
CSB-EP397530PZM
CSB-BP397530PZM
CSB-MP397530PZM
CSB-EP397530PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina High frequency lysogenization protein HflD homolog (hflD) CSB-YP397531PZM
CSB-EP397531PZM
CSB-BP397531PZM
CSB-MP397531PZM
CSB-EP397531PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP397532PZM
CSB-EP397532PZM
CSB-BP397532PZM
CSB-MP397532PZM
CSB-EP397532PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP397533PZM
CSB-EP397533PZM
CSB-BP397533PZM
CSB-MP397533PZM
CSB-EP397533PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Glutamate--tRNA ligase (gltX) CSB-YP397534PZM
CSB-EP397534PZM
CSB-BP397534PZM
CSB-MP397534PZM
CSB-EP397534PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cell division protein ZipA homolog (zipA), partial CSB-YP397535PZM
CSB-EP397535PZM
CSB-BP397535PZM
CSB-MP397535PZM
CSB-EP397535PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP397536PZM
CSB-EP397536PZM
CSB-BP397536PZM
CSB-MP397536PZM
CSB-EP397536PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP397537PZM
CSB-EP397537PZM
CSB-BP397537PZM
CSB-MP397537PZM
CSB-EP397537PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Lipid-A-disaccharide synthase (lpxB) CSB-YP397538PZM
CSB-EP397538PZM
CSB-BP397538PZM
CSB-MP397538PZM
CSB-EP397538PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP397539PZM
CSB-EP397539PZM
CSB-BP397539PZM
CSB-MP397539PZM
CSB-EP397539PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina GTPase Der (der) CSB-YP397540PZM
CSB-EP397540PZM
CSB-BP397540PZM
CSB-MP397540PZM
CSB-EP397540PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP397541PZM
CSB-EP397541PZM
CSB-BP397541PZM
CSB-MP397541PZM
CSB-EP397541PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Protein RnfH (rnfH) CSB-YP397542PZM
CSB-EP397542PZM
CSB-BP397542PZM
CSB-MP397542PZM
CSB-EP397542PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Adenine deaminase (Pmen_3706) CSB-YP397543PZM
CSB-EP397543PZM
CSB-BP397543PZM
CSB-MP397543PZM
CSB-EP397543PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UPF0250 protein Pmen_3793 (Pmen_3793) CSB-YP397544PZM
CSB-EP397544PZM
CSB-BP397544PZM
CSB-MP397544PZM
CSB-EP397544PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Gamma-glutamyl phosphate reductase (proA) CSB-YP397545PZM
CSB-EP397545PZM
CSB-BP397545PZM
CSB-MP397545PZM
CSB-EP397545PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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