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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas mendocina 50S ribosomal protein L15 (rplO) CSB-YP397546PZM
CSB-EP397546PZM
CSB-BP397546PZM
CSB-MP397546PZM
CSB-EP397546PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L16 (rplP) CSB-YP397547PZM
CSB-EP397547PZM
CSB-BP397547PZM
CSB-MP397547PZM
CSB-EP397547PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L23 (rplW) CSB-YP397548PZM
CSB-EP397548PZM
CSB-BP397548PZM
CSB-MP397548PZM
CSB-EP397548PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP397549PZM
CSB-EP397549PZM
CSB-BP397549PZM
CSB-MP397549PZM
CSB-EP397549PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L11 (rplK) CSB-YP397550PZM
CSB-EP397550PZM
CSB-BP397550PZM
CSB-MP397550PZM
CSB-EP397550PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP397551PZM
CSB-EP397551PZM
CSB-BP397551PZM
CSB-MP397551PZM
CSB-EP397551PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Thiosulfate sulfurtransferase glpE (glpE) CSB-YP397552PZM
CSB-EP397552PZM
CSB-BP397552PZM
CSB-MP397552PZM
CSB-EP397552PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP397553PZM
CSB-EP397553PZM
CSB-BP397553PZM
CSB-MP397553PZM
CSB-EP397553PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina RNA pyrophosphohydrolase (rppH) CSB-YP397554PZM
CSB-EP397554PZM
CSB-BP397554PZM
CSB-MP397554PZM
CSB-EP397554PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP397555PZM
CSB-EP397555PZM
CSB-BP397555PZM
CSB-MP397555PZM
CSB-EP397555PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP397556STQ
CSB-EP397556STQ
CSB-BP397556STQ
CSB-MP397556STQ
CSB-EP397556STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP397557STQ
CSB-EP397557STQ
CSB-BP397557STQ
CSB-MP397557STQ
CSB-EP397557STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L28 (rpmB) CSB-YP397558STQ
CSB-EP397558STQ
CSB-BP397558STQ
CSB-MP397558STQ
CSB-EP397558STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP397559STQ
CSB-EP397559STQ
CSB-BP397559STQ
CSB-MP397559STQ
CSB-EP397559STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP397560STQ
CSB-EP397560STQ
CSB-BP397560STQ
CSB-MP397560STQ
CSB-EP397560STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0114 protein Sputcn32_0673 (Sputcn32_0673), partial CSB-YP397561STQ1
CSB-EP397561STQ1
CSB-BP397561STQ1
CSB-MP397561STQ1
CSB-EP397561STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0251 protein Sputcn32_0687 (Sputcn32_0687) CSB-YP397562STQ
CSB-EP397562STQ
CSB-BP397562STQ
CSB-MP397562STQ
CSB-EP397562STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP397563STQ
CSB-EP397563STQ
CSB-BP397563STQ
CSB-MP397563STQ
CSB-EP397563STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP397564STQ
CSB-EP397564STQ
CSB-BP397564STQ
CSB-MP397564STQ
CSB-EP397564STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP397565STQ
CSB-EP397565STQ
CSB-BP397565STQ
CSB-MP397565STQ
CSB-EP397565STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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