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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Metallosphaera sedula Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP396604MNM
CSB-EP396604MNM
CSB-BP396604MNM
CSB-MP396604MNM
CSB-EP396604MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula DNA/RNA-binding protein Alba (albA) CSB-YP396605MNM
CSB-EP396605MNM
CSB-BP396605MNM
CSB-MP396605MNM
CSB-EP396605MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Ribonuclease Z (rnz) CSB-YP396606MNM
CSB-EP396606MNM
CSB-BP396606MNM
CSB-MP396606MNM
CSB-EP396606MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Methionine--tRNA ligase (metG), partial CSB-YP396607MNM
CSB-EP396607MNM
CSB-BP396607MNM
CSB-MP396607MNM
CSB-EP396607MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula V-type ATP synthase subunit D (atpD) CSB-YP396608MNM
CSB-EP396608MNM
CSB-BP396608MNM
CSB-MP396608MNM
CSB-EP396608MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Leucine--tRNA ligase 2 (leuS2), partial CSB-YP396609MNM
CSB-EP396609MNM
CSB-BP396609MNM
CSB-MP396609MNM
CSB-EP396609MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Aspartate carbamoyltransferase (pyrB) CSB-YP396610MNM
CSB-EP396610MNM
CSB-BP396610MNM
CSB-MP396610MNM
CSB-EP396610MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Putative adenylate kinase (Msed_2172) CSB-YP396611MNM
CSB-EP396611MNM
CSB-BP396611MNM
CSB-MP396611MNM
CSB-EP396611MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 30S ribosomal protein S15P/S13e (rps15p) CSB-YP396612MNM
CSB-EP396612MNM
CSB-BP396612MNM
CSB-MP396612MNM
CSB-EP396612MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosome maturation factor RimP (rimP) CSB-YP396613BPF
CSB-EP396613BPF
CSB-BP396613BPF
CSB-MP396613BPF
CSB-EP396613BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP396614BPF
CSB-EP396614BPF
CSB-BP396614BPF
CSB-MP396614BPF
CSB-EP396614BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Tryptophan synthase beta chain (trpB) CSB-YP396615BPF
CSB-EP396615BPF
CSB-BP396615BPF
CSB-MP396615BPF
CSB-EP396615BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Protein grpE (grpE) CSB-YP396616BPF
CSB-EP396616BPF
CSB-BP396616BPF
CSB-MP396616BPF
CSB-EP396616BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. tRNA modification GTPase MnmE (mnmE) CSB-YP396617BPF
CSB-EP396617BPF
CSB-BP396617BPF
CSB-MP396617BPF
CSB-EP396617BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP396618BPF
CSB-EP396618BPF
CSB-BP396618BPF
CSB-MP396618BPF
CSB-EP396618BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0434 protein BRADO0313 (BRADO0313) CSB-YP396619BPF
CSB-EP396619BPF
CSB-BP396619BPF
CSB-MP396619BPF
CSB-EP396619BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosome maturation factor RimM (rimM) CSB-YP396620BPF
CSB-EP396620BPF
CSB-BP396620BPF
CSB-MP396620BPF
CSB-EP396620BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Protein translocase subunit SecA (secA), partial CSB-YP396621BPF
CSB-EP396621BPF
CSB-BP396621BPF
CSB-MP396621BPF
CSB-EP396621BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Carboxylate-amine ligase BRADO0861 (BRADO0861) CSB-YP396622BPF
CSB-EP396622BPF
CSB-BP396622BPF
CSB-MP396622BPF
CSB-EP396622BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP396623BPF
CSB-EP396623BPF
CSB-BP396623BPF
CSB-MP396623BPF
CSB-EP396623BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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