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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP396424RLG
CSB-EP396424RLG
CSB-BP396424RLG
CSB-MP396424RLG
CSB-EP396424RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP396425RLG
CSB-EP396425RLG
CSB-BP396425RLG
CSB-MP396425RLG
CSB-EP396425RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Bifunctional protein GlmU (glmU) CSB-YP396426RLG
CSB-EP396426RLG
CSB-BP396426RLG
CSB-MP396426RLG
CSB-EP396426RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides GTPase Der (der) CSB-YP396427RLG
CSB-EP396427RLG
CSB-BP396427RLG
CSB-MP396427RLG
CSB-EP396427RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Histidinol-phosphate aminotransferase (hisC) CSB-YP396428RLG
CSB-EP396428RLG
CSB-BP396428RLG
CSB-MP396428RLG
CSB-EP396428RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP396429RLG
CSB-EP396429RLG
CSB-BP396429RLG
CSB-MP396429RLG
CSB-EP396429RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Alanine--tRNA ligase (alaS), partial CSB-YP396430RLG
CSB-EP396430RLG
CSB-BP396430RLG
CSB-MP396430RLG
CSB-EP396430RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L35 (rpmI) CSB-YP396431RLG
CSB-EP396431RLG
CSB-BP396431RLG
CSB-MP396431RLG
CSB-EP396431RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S13 (rpsM) CSB-YP396432RLG
CSB-EP396432RLG
CSB-BP396432RLG
CSB-MP396432RLG
CSB-EP396432RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L18 (rplR) CSB-YP396433RLG
CSB-EP396433RLG
CSB-BP396433RLG
CSB-MP396433RLG
CSB-EP396433RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L14 (rplN) CSB-YP396434RLG
CSB-EP396434RLG
CSB-BP396434RLG
CSB-MP396434RLG
CSB-EP396434RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L2 (rplB) CSB-YP396435RLG
CSB-EP396435RLG
CSB-BP396435RLG
CSB-MP396435RLG
CSB-EP396435RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S12 (rpsL) CSB-YP396436RLG
CSB-EP396436RLG
CSB-BP396436RLG
CSB-MP396436RLG
CSB-EP396436RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP396437RLG
CSB-EP396437RLG
CSB-BP396437RLG
CSB-MP396437RLG
CSB-EP396437RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribonuclease PH (rph) CSB-YP396438RLG
CSB-EP396438RLG
CSB-BP396438RLG
CSB-MP396438RLG
CSB-EP396438RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribulose bisphosphate carboxylase large chain 1 (cbbL1) CSB-YP396439RLG
CSB-EP396439RLG
CSB-BP396439RLG
CSB-MP396439RLG
CSB-EP396439RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Chaperone protein DnaJ (dnaJ) CSB-YP396440RLG
CSB-EP396440RLG
CSB-BP396440RLG
CSB-MP396440RLG
CSB-EP396440RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosome-binding factor A (rbfA) CSB-YP396441RLG
CSB-EP396441RLG
CSB-BP396441RLG
CSB-MP396441RLG
CSB-EP396441RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides RNA pyrophosphohydrolase (rppH) CSB-YP396442RLG
CSB-EP396442RLG
CSB-BP396442RLG
CSB-MP396442RLG
CSB-EP396442RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Indole-3-glycerol phosphate synthase (trpC) CSB-YP396443RLG
CSB-EP396443RLG
CSB-BP396443RLG
CSB-MP396443RLG
CSB-EP396443RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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