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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salinispora tropica Phosphoglycerate kinase (pgk) CSB-YP396464STD
CSB-EP396464STD
CSB-BP396464STD
CSB-MP396464STD
CSB-EP396464STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP396465CVX
CSB-EP396465CVX
CSB-BP396465CVX
CSB-MP396465CVX
CSB-EP396465CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Phosphoglucosamine mutase (glmM) CSB-YP396466CVX
CSB-EP396466CVX
CSB-BP396466CVX
CSB-MP396466CVX
CSB-EP396466CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Glycogen synthase (glgA) CSB-YP396467CVX
CSB-EP396467CVX
CSB-BP396467CVX
CSB-MP396467CVX
CSB-EP396467CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0182 protein Csac_0864 (Csac_0864), partial CSB-YP396468CVX
CSB-EP396468CVX
CSB-BP396468CVX
CSB-MP396468CVX
CSB-EP396468CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP396469CVX
CSB-EP396469CVX
CSB-BP396469CVX
CSB-MP396469CVX
CSB-EP396469CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP396470CVX
CSB-EP396470CVX
CSB-BP396470CVX
CSB-MP396470CVX
CSB-EP396470CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP396471CVX
CSB-EP396471CVX
CSB-BP396471CVX
CSB-MP396471CVX
CSB-EP396471CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0042 nucleotide-binding protein Csac_1160 (Csac_1160) CSB-YP396472CVX
CSB-EP396472CVX
CSB-BP396472CVX
CSB-MP396472CVX
CSB-EP396472CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Peptidyl-tRNA hydrolase (pth) CSB-YP396473CVX
CSB-EP396473CVX
CSB-BP396473CVX
CSB-MP396473CVX
CSB-EP396473CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 10 kDa chaperonin (groS) CSB-YP396474CVX
CSB-EP396474CVX
CSB-BP396474CVX
CSB-MP396474CVX
CSB-EP396474CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP396475CVX
CSB-EP396475CVX
CSB-BP396475CVX
CSB-MP396475CVX
CSB-EP396475CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus D-alanine--D-alanine ligase (ddl) CSB-YP396476CVX
CSB-EP396476CVX
CSB-BP396476CVX
CSB-MP396476CVX
CSB-EP396476CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L9 (rplI) CSB-YP396477CVX
CSB-EP396477CVX
CSB-BP396477CVX
CSB-MP396477CVX
CSB-EP396477CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP396478CVX
CSB-EP396478CVX
CSB-BP396478CVX
CSB-MP396478CVX
CSB-EP396478CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP396479CVX
CSB-EP396479CVX
CSB-BP396479CVX
CSB-MP396479CVX
CSB-EP396479CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0297 protein Csac_1773 (Csac_1773) CSB-YP396480CVX
CSB-EP396480CVX
CSB-BP396480CVX
CSB-MP396480CVX
CSB-EP396480CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L32 (rpmF) CSB-YP396481CVX
CSB-EP396481CVX
CSB-BP396481CVX
CSB-MP396481CVX
CSB-EP396481CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Maf-like protein Csac_1865 (Csac_1865) CSB-YP396482CVX
CSB-EP396482CVX
CSB-BP396482CVX
CSB-MP396482CVX
CSB-EP396482CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Arginine repressor (argR) CSB-YP396483CVX
CSB-EP396483CVX
CSB-BP396483CVX
CSB-MP396483CVX
CSB-EP396483CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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