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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides N (2)-fixation sustaining protein CowN CSB-YP396444RLG
CSB-EP396444RLG
CSB-BP396444RLG
CSB-MP396444RLG
CSB-EP396444RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica DNA replication and repair protein recF (recF) CSB-YP396445STD
CSB-EP396445STD
CSB-BP396445STD
CSB-MP396445STD
CSB-EP396445STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Methionine--tRNA ligase (metG), partial CSB-YP396446STD
CSB-EP396446STD
CSB-BP396446STD
CSB-MP396446STD
CSB-EP396446STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ferredoxin--NADP reductase (Strop_0871) CSB-YP396447STD
CSB-EP396447STD
CSB-BP396447STD
CSB-MP396447STD
CSB-EP396447STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica SsrA-binding protein (smpB) CSB-YP396448STD
CSB-EP396448STD
CSB-BP396448STD
CSB-MP396448STD
CSB-EP396448STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP396449STD
CSB-EP396449STD
CSB-BP396449STD
CSB-MP396449STD
CSB-EP396449STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Dihydroorotate dehydrogenase (quinone) CSB-YP396450STD
CSB-EP396450STD
CSB-BP396450STD
CSB-MP396450STD
CSB-EP396450STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica ATP phosphoribosyltransferase (hisG) CSB-YP396451STD
CSB-EP396451STD
CSB-BP396451STD
CSB-MP396451STD
CSB-EP396451STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Proteasome-associated ATPase (arc), partial CSB-YP396452STD
CSB-EP396452STD
CSB-BP396452STD
CSB-MP396452STD
CSB-EP396452STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Pup--protein ligase (pafA) CSB-YP396453STD
CSB-EP396453STD
CSB-BP396453STD
CSB-MP396453STD
CSB-EP396453STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L33 1 (rpmG1) CSB-YP396454STD
CSB-EP396454STD
CSB-BP396454STD
CSB-MP396454STD
CSB-EP396454STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP396455STD
CSB-EP396455STD
CSB-BP396455STD
CSB-MP396455STD
CSB-EP396455STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP396456STD
CSB-EP396456STD
CSB-BP396456STD
CSB-MP396456STD
CSB-EP396456STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Probable rRNA maturation factor (Strop_3452) CSB-YP396457STD
CSB-EP396457STD
CSB-BP396457STD
CSB-MP396457STD
CSB-EP396457STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Bifunctional protein FolD 2 (folD2) CSB-YP396458STD
CSB-EP396458STD
CSB-BP396458STD
CSB-MP396458STD
CSB-EP396458STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L5 (rplE) CSB-YP396459STD
CSB-EP396459STD
CSB-BP396459STD
CSB-MP396459STD
CSB-EP396459STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 30S ribosomal protein S3 (rpsC) CSB-YP396460STD
CSB-EP396460STD
CSB-BP396460STD
CSB-MP396460STD
CSB-EP396460STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L11 (rplK) CSB-YP396461STD
CSB-EP396461STD
CSB-BP396461STD
CSB-MP396461STD
CSB-EP396461STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP396462STD
CSB-EP396462STD
CSB-BP396462STD
CSB-MP396462STD
CSB-EP396462STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Pantothenate synthetase (panC) CSB-YP396463STD
CSB-EP396463STD
CSB-BP396463STD
CSB-MP396463STD
CSB-EP396463STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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