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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. Ribonuclease Z (rnz) CSB-YP396624BPF
CSB-EP396624BPF
CSB-BP396624BPF
CSB-MP396624BPF
CSB-EP396624BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable 5-dehydro-4-deoxyglucarate dehydratase (BRADO1565) CSB-YP396625BPF
CSB-EP396625BPF
CSB-BP396625BPF
CSB-MP396625BPF
CSB-EP396625BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP396626BPF
CSB-EP396626BPF
CSB-BP396626BPF
CSB-MP396626BPF
CSB-EP396626BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Chorismate synthase (aroC) CSB-YP396627BPF
CSB-EP396627BPF
CSB-BP396627BPF
CSB-MP396627BPF
CSB-EP396627BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Error-prone DNA polymerase (dnaE2), partial CSB-YP396628BPF
CSB-EP396628BPF
CSB-BP396628BPF
CSB-MP396628BPF
CSB-EP396628BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP396629BPF
CSB-EP396629BPF
CSB-BP396629BPF
CSB-MP396629BPF
CSB-EP396629BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L3 (rplC) CSB-YP396630BPF
CSB-EP396630BPF
CSB-BP396630BPF
CSB-MP396630BPF
CSB-EP396630BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L14 (rplN) CSB-YP396631BPF
CSB-EP396631BPF
CSB-BP396631BPF
CSB-MP396631BPF
CSB-EP396631BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S5 (rpsE) CSB-YP396632BPF
CSB-EP396632BPF
CSB-BP396632BPF
CSB-MP396632BPF
CSB-EP396632BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S11 (rpsK) CSB-YP396633BPF
CSB-EP396633BPF
CSB-BP396633BPF
CSB-MP396633BPF
CSB-EP396633BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Polyphosphate kinase (ppk), partial CSB-YP396634BPF
CSB-EP396634BPF
CSB-BP396634BPF
CSB-MP396634BPF
CSB-EP396634BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP396635BPF
CSB-EP396635BPF
CSB-BP396635BPF
CSB-MP396635BPF
CSB-EP396635BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Deoxyguanosinetriphosphate triphosphohydrolase-like protein (BRADO4053) CSB-YP396636BPF
CSB-EP396636BPF
CSB-BP396636BPF
CSB-MP396636BPF
CSB-EP396636BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Glutamate--tRNA ligase (gltX) CSB-YP396637BPF
CSB-EP396637BPF
CSB-BP396637BPF
CSB-MP396637BPF
CSB-EP396637BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP396638BPF
CSB-EP396638BPF
CSB-BP396638BPF
CSB-MP396638BPF
CSB-EP396638BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Putative Holliday junction resolvase (BRADO4497) CSB-YP396639BPF
CSB-EP396639BPF
CSB-BP396639BPF
CSB-MP396639BPF
CSB-EP396639BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Cobyric acid synthase (cobQ) CSB-YP396640BPF
CSB-EP396640BPF
CSB-BP396640BPF
CSB-MP396640BPF
CSB-EP396640BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Glutathione-dependent formaldehyde-activating enzyme (gfa) CSB-YP396641BPF
CSB-EP396641BPF
CSB-BP396641BPF
CSB-MP396641BPF
CSB-EP396641BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP396642BPF
CSB-EP396642BPF
CSB-BP396642BPF
CSB-MP396642BPF
CSB-EP396642BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP phosphoribosyltransferase (hisG) CSB-YP396643BPF
CSB-EP396643BPF
CSB-BP396643BPF
CSB-MP396643BPF
CSB-EP396643BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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