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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orientia tsutsugamushi 30S ribosomal protein S16 (rpsP) CSB-YP396665ODJ
CSB-EP396665ODJ
CSB-BP396665ODJ
CSB-MP396665ODJ
CSB-EP396665ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP396666ODJ
CSB-EP396666ODJ
CSB-BP396666ODJ
CSB-MP396666ODJ
CSB-EP396666ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP396667ODJ
CSB-EP396667ODJ
CSB-BP396667ODJ
CSB-MP396667ODJ
CSB-EP396667ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP396668ODJ
CSB-EP396668ODJ
CSB-BP396668ODJ
CSB-MP396668ODJ
CSB-EP396668ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Histidine--tRNA ligase (hisS) CSB-YP396669ODJ
CSB-EP396669ODJ
CSB-BP396669ODJ
CSB-MP396669ODJ
CSB-EP396669ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi DNA repair protein recO (recO) CSB-YP396670ODJ
CSB-EP396670ODJ
CSB-BP396670ODJ
CSB-MP396670ODJ
CSB-EP396670ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP396671ODJ
CSB-EP396671ODJ
CSB-BP396671ODJ
CSB-MP396671ODJ
CSB-EP396671ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Chaperone protein DnaK (dnaK), partial CSB-YP396672CWT
CSB-EP396672CWT
CSB-BP396672CWT
CSB-MP396672CWT
CSB-EP396672CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Serine--tRNA ligase (serS) CSB-YP396673CWT
CSB-EP396673CWT
CSB-BP396673CWT
CSB-MP396673CWT
CSB-EP396673CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Xylose isomerase (xylA) CSB-YP396674CWT
CSB-EP396674CWT
CSB-BP396674CWT
CSB-MP396674CWT
CSB-EP396674CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis SsrA-binding protein (smpB) CSB-YP396675CWT
CSB-EP396675CWT
CSB-BP396675CWT
CSB-MP396675CWT
CSB-EP396675CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis DNA ligase (ligA), partial CSB-YP396676CWT
CSB-EP396676CWT
CSB-BP396676CWT
CSB-MP396676CWT
CSB-EP396676CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis GTPase obg (obg), partial CSB-YP396677CWT
CSB-EP396677CWT
CSB-BP396677CWT
CSB-MP396677CWT
CSB-EP396677CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Elongation factor 4 (lepA), partial CSB-YP396678CWT
CSB-EP396678CWT
CSB-BP396678CWT
CSB-MP396678CWT
CSB-EP396678CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L31 type B (rpmE2) CSB-YP396679CWT
CSB-EP396679CWT
CSB-BP396679CWT
CSB-MP396679CWT
CSB-EP396679CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP396680CWT
CSB-EP396680CWT
CSB-BP396680CWT
CSB-MP396680CWT
CSB-EP396680CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP396681CWT
CSB-EP396681CWT
CSB-BP396681CWT
CSB-MP396681CWT
CSB-EP396681CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Protein MraZ (mraZ) CSB-YP396682CWT
CSB-EP396682CWT
CSB-BP396682CWT
CSB-MP396682CWT
CSB-EP396682CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S15 (rpsO) CSB-YP396683CWT
CSB-EP396683CWT
CSB-BP396683CWT
CSB-MP396683CWT
CSB-EP396683CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP396684CWT
CSB-EP396684CWT
CSB-BP396684CWT
CSB-MP396684CWT
CSB-EP396684CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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